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Table 2 Immunoglobulin titres (IgG plus IgM mean log titers and IgG2a/IgG1 log ratios) in mice, measured at week 4 after immunization with bacterins, either physically mixed just with EDA or bound to EDA (EDAvidin) by streptavidin-biotin (BEDA) interaction

From: The extradomain a of fibronectin enhances the efficacy of lipopolysaccharide defective Salmonella bacterins as vaccines in mice

Inoculation group

Log10 IgM + IgG

Log10 IgG2a/IgG1 ratio

 

(mean ± SD) a

(mean ± SD) a

SE-wt live

3.45 ± 0.17

1.18 ± 0.26

SEΔwaaL live

3.60 ± 0.73

1.21 ± 0.10

SEΔgal live

2.02 ± 0.45

1.05 ± 0.07

B-SEΔwaaL + EDA

2.54 ± 0.38

1.05 ± 0.20

B-SEΔgal + EDA

2.69 ± 0.42

0.85 ± 0.12

BEDA-SEΔwaaL

2.77 ± 0.51

1.45 ± 0.20

BEDA-SEΔgal

3.07 ± 0.51

0.74 ± 0.09

  1. a Serum titre was defined as the reciprocal of the highest serum dilution showing a mean O.D. value equal to or higher than that of the negative control sera (n = 4) obtained from mice inoculated with PBS, plus 3 times the SD. Mean and SD (n = 4) represent the data obtained in triplicate.
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Veterinary Research

ISSN: 1297-9716