Characterization of homologous and heterologous adaptive immune responses in porcine reproductive and respiratory syndrome virus infection

Table 8 Serum levels of TGF-β after challenge with PRRSV at 84 days after the initial inoculation

	Corrected† TGF-ß levels in serum (pg/mL) N° responding pigs; Average ± standard deviation
Experiment 1 Group	Days post-challenge
	1	2	3	7	14
C1 + B	0/2	0/2	1/2	1/2	1/2
	N.A.	N.A.	74 ± N.A.	113 ± N.A.	23 ± 18
A + A	3/4	3/4	4/4	4/4	4/4
	50 ± 10	67 ± 17	46 ± 60	93 ± 14	152 ± 110
A + B	3/4	2/4	2/4	4/4^a	4/4
	57 ± 60	115 ± 60	109 ± 50	99 ± 59	144 ± 90
Experiment 2 Group	Days post-challenge
	1	2	3*	7*	14
C2 + A	1/3	1/3	0/3	1/3	1/3
	57 ± N.A.	149 ± N.A.	N.A.	17 ± N.A.	33 ± N.A.
B + B	4/4	4/4	4/4	4/4	4/4
	42 ± 29	42 ± 16	99 ± 41	66 ± 66	109 ± 140
B + A	2/3	2/3	0/3	0/3^b	1/3
	106 ± 34	109 ± 61	N.A.	N.A	37 ± N.A.

† In order to account the pre-2nd challenge levels of a given cytokine, values (pg/mL) obtained with samples of day 0 (84 pi) (C1 + B:141 ± 20; A + A:58 ± 29; A + B:86 ± 63; C2 + A:124 ± 107; B + B:137 ± 14; B + A:1036 ± 1701) were subtracted to the values obtained at days 1, 2, 3, 7 or 14, respectively for producing a corrected value (Corrected value for cytokine X = Concentration of cytokine X _{day n} - Concentration of cytokine X _{day 0}).
N.A. not applicable. Symbols (*p ≤ 0.05) show days for which statistical differences between groups with regards to the proportion of positive pigs were observed. Letters indicate statistically significant differences for the proportion of responding pigs for similar groups between experiments; i.e. naïve pigs inoculated with 3267 (C1 + B) vs. Naïve pigs inoculated with 3262 (C2 + A). In this case, a,b correspond to p ≤ 0.05.

ISSN: 1297-9716