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Table 4 Detection of virus in sera by isolation in porcine alveolar macrophages and by nested RT-PCR after the second challenge with PRRSV

From: Characterization of homologous and heterologous adaptive immune responses in porcine reproductive and respiratory syndrome virus infection

   

Days post-2nd challenge Positive/total Viral titre ± standard deviation

Experiment

Group

Assay

+7 (91 pi)

+14 (98 pi)

1

C1 + B

Viral isolation

2/2

2/2

3.2 ± 0.6

2.0 ± 0.0

nRT-PCR

2/2

2/2

A + A

Viral isolation

0/4

0/4

nRT-PCR

4/4

1/4

A + B

Viral isolation

0/4

0/4

  

nRT-PCR

4/4

0/4

2

C2 + A

Viral isolation

3/3

2/3

3.4 ± 0.9

2.2 ± 0.1

nRT-PCR

3/3

3/3

B + B

Viral isolation

0/4

0/4

nRT-PCR

0/4*

0/4

B + A

Viral isolation

0/4

0/4

  

nRT-PCR

0/4*

0/4

  1. *Asterisks indicate statistical differences in the proportion of pigs found to be positive by RT-PCR in comparable groups of experiments 1 and 2 (i.e. homologous A + A versus homologous B + B).
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Veterinary Research

ISSN: 1297-9716