Veterinary Research

Table 3 IHC- and histopathology mean scores.

From: Immunohistochemical detection of piscine reovirus (PRV) in hearts of Atlantic salmon coincide with the course of heart and skeletal muscle inflammation (HSMI)

A. Inoculated group
	IHC Anti-σ1		IHC Anti-μ1C		Histopathology
Wpi	Leuk.	Myocyte	Leuk.	Myocyte	Epicard	Myocard
2 wpi	0 ± 0	0 ± 0	0 ± 0	0 ± 0	0.20 ± 0.12	0 ± 0
4 wpi	1.00 ± 0.71	0 ± 0	0.60 ± 0.55	0 ± 0	0.14 ± 0.19	0 ± 0
6 wpi	1.40 ± 0.89	1.40 ± 1.14	0.60 ± 0.55	1.40 ± 1.52	1.18 ± 0.56	0.16 ± 0.26
8 wpi	0.60 ± 0.55	1.60 ± 0.55	0.20 ± 0.45	1.40 ± 0.55	1.68 ± 0.45	0.80 ± 0.49
10 wpi	0 ± 0	0.40 ± 0.55	0 ± 0	0.60 ± 0.55	1.60 ± 0.57	0.60 ± 0.55
12 wpi	0 ± 0	0.20 ± 0.45	0 ± 0	0.20 ± 0.45	0.62 ± 0.29	0.94 ± 0.17
B. Cohabitant group
	IHC Anti-σ1		IHC Anti-μ1C		Histopathology
Wpi	Leuk.	Myocyte	Leuk.	Myocyte	Epicard	Myocard
6 wpi	0 ± 0	0 ± 0	0 ± 0	0 ± 0	0.34 ± 0.16	0.06 ± 0.06
8 wpi	1.80 ± 1.10	0 ± 0	1.40 ± 0.89	0 ± 0	0.58 ± 0.37	0 ± 0
10 wpi	1.40 ± 0.89	3.20 ± 1.64	0.80 ± 0.83	3.20 ± 1.30	1.90 ± 0.60	0.56 ± 0.46
12 wpi	0 ± 0	1.80 ± 0.84	0 ± 0	2.00 ± 1.00	1.94 ± 0.54	1.7 ± 0.43

Data represent mean scores (± SD) for IHC staining and histopathological changes at each time of sampling (n = 5) shown for the inoculated (A) and cohabitant group (B). The IHC staining was performed with both Anti-σ1 and Anti-μ1C and scored (0-5) for positive leukocyte-like cells and cardiomyocytes. The histological changes were scored (0-3) for epicardial- and myocardial changes.

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ISSN: 1297-9716

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