|
A. Inoculated group
|
|---|
| |
IHC Anti-σ1
|
IHC Anti-μ1C
|
Histopathology
|
|
Wpi
|
Leuk.
|
Myocyte
|
Leuk.
|
Myocyte
|
Epicard
|
Myocard
|
|
2 wpi
|
0 ± 0
|
0 ± 0
|
0 ± 0
|
0 ± 0
|
0.20 ± 0.12
|
0 ± 0
|
|
4 wpi
|
1.00 ± 0.71
|
0 ± 0
|
0.60 ± 0.55
|
0 ± 0
|
0.14 ± 0.19
|
0 ± 0
|
|
6 wpi
|
1.40 ± 0.89
|
1.40 ± 1.14
|
0.60 ± 0.55
|
1.40 ± 1.52
|
1.18 ± 0.56
|
0.16 ± 0.26
|
|
8 wpi
|
0.60 ± 0.55
|
1.60 ± 0.55
|
0.20 ± 0.45
|
1.40 ± 0.55
|
1.68 ± 0.45
|
0.80 ± 0.49
|
|
10 wpi
|
0 ± 0
|
0.40 ± 0.55
|
0 ± 0
|
0.60 ± 0.55
|
1.60 ± 0.57
|
0.60 ± 0.55
|
|
12 wpi
|
0 ± 0
|
0.20 ± 0.45
|
0 ± 0
|
0.20 ± 0.45
|
0.62 ± 0.29
|
0.94 ± 0.17
|
|
B. Cohabitant group
|
| |
IHC Anti-σ1
|
IHC Anti-μ1C
|
Histopathology
|
|
Wpi
|
Leuk.
|
Myocyte
|
Leuk.
|
Myocyte
|
Epicard
|
Myocard
|
|
6 wpi
|
0 ± 0
|
0 ± 0
|
0 ± 0
|
0 ± 0
|
0.34 ± 0.16
|
0.06 ± 0.06
|
|
8 wpi
|
1.80 ± 1.10
|
0 ± 0
|
1.40 ± 0.89
|
0 ± 0
|
0.58 ± 0.37
|
0 ± 0
|
|
10 wpi
|
1.40 ± 0.89
|
3.20 ± 1.64
|
0.80 ± 0.83
|
3.20 ± 1.30
|
1.90 ± 0.60
|
0.56 ± 0.46
|
|
12 wpi
|
0 ± 0
|
1.80 ± 0.84
|
0 ± 0
|
2.00 ± 1.00
|
1.94 ± 0.54
|
1.7 ± 0.43
|
- Data represent mean scores (± SD) for IHC staining and histopathological changes at each time of sampling (n = 5) shown for the inoculated (A) and cohabitant group (B). The IHC staining was performed with both Anti-σ1 and Anti-μ1C and scored (0-5) for positive leukocyte-like cells and cardiomyocytes. The histological changes were scored (0-3) for epicardial- and myocardial changes.
