Figure 2

IHC and histopathology for the inoculated group. Immunostaining of heart tissue sections and graphical presentation of the IHC- and histopathological scores from the inoculated group. (A) Blood clot in heart 4 wpi: PRV antigen detected with σ1-antibodies in a single leukocyte-like cell observed as red cytoplasmatic staining. Error bar 15 μm. (B) Heart ventricle 6 wpi: Immunostained cardiomyocytes detected with μ1c-antibodies in the outer part of the compact layer (arrow). Error bar 50 μm. (C) Heart ventricle 8 wpi: Scattered immunostained cardiomyocytes (arrow) observed in the inner part of compact layer and in the spongy layer. No positive cells detected within the inflamed outer compactum in the top half of the picture. Section stained with σ1-antibodies. Error bar 100 μm. (D) The graph illustrates the IHC-staining with σ1 antiserum and the histopathological changes at each time of sampling (Wpi). IHC-scores (left Y-axis) are shown for leukocyte-like cells (blue) and cardiomyocytes (red). Histopathological score (right Y-axis) are presented for epicardial-(gray) and ventricular changes (black). All bars represent mean values (+SD) for each group (n = 5).