Figure 5

Histological analysis of mice after challenge with S. aureus. (A) Liver histopathology from mice challenged with S. aureus after vaccination with PLGA-rSEA. (B) Liver histopathology of unvaccinated mice challenged with S. aureus. The black arrow indicates moderate infiltration of mononuclear cells around the central veins (C) Spleen histopathology of mice immunized with PLGA-rSEA. (D) Spleen histopathology of unvaccinated mice. (E) Kidney histopathology of mice immunized with PLGA-rSEA. The black arrow indicates erythrocytes and macrophages in the white pulp. (F) Kidney histopathology of unvaccinated mice. BALB/c mice were vaccinated and then challenged i.p. with 10⁸ CFU of S. aureus six weeks after immunization. Mice were sacrificed on day 9 after challenge for this analysis. Tissues were stained with hematoxylin and eosin. Black arrows indicate the necrotic and scaled of the tubular structure.