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Table 1 PCR primer pairs, origin and features of apfA, apxA and tbpB genes and corresponding proteins

From: Type IV fimbrial subunit protein ApfA contributes to protection against porcine pleuropneumonia

Gene1

A. pleuropneumoniae

serotype

PCR primer pair (5' → 3')2

Gene length

(bps)

Protein MW

(kDa)3

apfA

7

CCGTCGAC ATGTCATATAACAGTTATAC

CCGCGGCCGC ATTTGATGCGCAGAAATTT

342

14

apxIA

1

GGGTCGAC ACGCTAACTCTCAGCTCGATA

GGGCGGCCGC AGCTGCTTGTGCTAAAGAA

3069

113

apxIIA

7

CCGTCGAC ACTCAAAAATCACTTTGTCATCA

CCGCGGCCGC AGCGGCTCTAGCTAATTG

2871

105

apxIIIA

2

GGGTCGAC ACAGTACTTGGTCAAGCATGTT

GGGCGGCCGC AGCTGCTCTAGCTAGGTTA

3159

115

apxIVA

3

CCGTCGAC ACACAAAATTAACTATGCAAGA

CCGCGGCCGC TAAAGCAGCTGTTAAGCTATT

5418

205

tbpB

7

CCGTCGAC ACTCTGGCGGAAAAGGAAGTT

CCGCGGCCGC TTTTTTTACTTGTTGTTTTGCA

1644

60

  1. 1Sequences of genes were acquired from GenBank (apfA, AY235719.1; apxIA, AF240779; apxIIA, CP001091; apxIIIA, L12145; apxIVA, AF030511; tbpB, U16017).
  2. 2All oligonucleotides were synthesized by Generi Biotech, Hradec Kralove, Czech Republic. First oligonucleotide represents forward PCR primer, second reverse PCR primer. Sal I and Not I recognition sites are marked in bold and underlined.
  3. 3The approximate protein molecular weight was calculated using the Compute pI/Mw tool (ExPASy).