Comparison of mature NK-lysins and granulysin from farm animals. Primary structure alignment of mature NK-lysins from the horse and pig and granulysin from cattle. Conserved and semi-conserved amino-acid residues with similar properties are highlighted in light grey, cysteine residues in dark grey. Similar amino acids were defined as follows: (A, G); (S, T); (E, D); (R, K, H); (Q, N); (V, I, L, M); (Y, F); (W); (P); (C) . The arrow indicates an additional cysteine residue only present in the equine sequence. Gaps introduced to maximize amino-acid alignments are indicated by hyphens. The percent similarity to equine NK-lysin is given at the end of the pig/cattle sequence.