MALDI-TOF peptide mass fingerprinting analysis. Figure 3A: Coomassie brilliant blue G250 stained gel of BNP-dam serum precipitated MDBK lysate. Bands of interest (44 kDa and 12 kDa, arrows) were excised and analyzed by MALDI-TOF peptide mass fingerprinting. Figure 3B: MHC I [EMBL:AAZ73457.1 (http://www.ebi.ac.uk/ena/data/view/AAZ73457)] sequence coverage of detected peptides was 49.2% (blue bars). Two peptide masses (997.6 Da; 1127.6 Da) derived from the 12 kDa band, matched to tryptic peptides 103HVTLEQPR and 68SEQSDLSFSK of bovine β-2-microglobulin [EMBL:AAI18353.1 (http://www.ebi.ac.uk/ena/data/view/AAI18353)].