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Figure 6 | Veterinary Research

Figure 6

From: Malignant catarrhal fever induced by Alcelaphine herpesvirus 1 is characterized by an expansion of activated CD3+CD8+CD4- T cells expressing a cytotoxic phenotype in both lymphoid and non-lymphoid tissues

Figure 6

IFN-γ production by CD8+ T cells isolated from lymphoid and non-lymphoid organs during WD-MCF in rabbits. At time of euthanasia, analysis by multi-colour flow cytometry was conducted on PBMC and mononuclear leukocytes isolated from pLN, spleen, liver, lung and kidney of mock-infected or AlHV-1-infected rabbits developing WD-MCF. Cells were cultured with PMA/ionomycin and brefeldin A for 4 h and analyzed for ex vivo IFN-γ production capability by triple-colour staining. (A) Flow cytometry dot plots obtained by multi-colour staining of pLN cells of one representative rabbit of each group. A first gate was placed on T-lymphocytes (left column) to measure IFN-γ production in CD8- or CD8+ T cells (right column). (B) IFN-γ production in CD8- or CD8+ T cells in PBMC over time after infection. Data represent individual measurements. (C) IFN-γ production in CD8- or CD8+ T cells at time of euthanasia in PBMC and mononuclear leukocytes isolated in pLN, spleen, liver, lung and kidney. The percentages of T-lymphocytes-positive cells in the kidney of mock-infected animals were below 3% and were therefore not plotted. Data are representative of two independent experiments (n = 3). Bars show means ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001.

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Veterinary Research

ISSN: 1297-9716