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Figure 1 | Veterinary Research

Figure 1

From: Malignant catarrhal fever induced by Alcelaphine herpesvirus 1 is characterized by an expansion of activated CD3+CD8+CD4- T cells expressing a cytotoxic phenotype in both lymphoid and non-lymphoid tissues

Figure 1

Analysis of rabbit peripheral blood mononuclear cells by multi-colour flow cytometry. PBMC were isolated from K3EDTA-sampled blood of naive rabbits before five-colour flow cytometry analysis. Specific detection of monocytes, B cells and T cell subsets consisted in a 3-step staining procedure. PBMC were first stained with anti-rabbit IgM (or mouse IgG1 isotype control), anti-rabbit CD8 (or mouse IgG1 isotype control) and anti-rabbit CD4 (or mouse IgG2a isotype control). Stainings were revealed with PE-conjugated rat anti-IgG1 or biotinylated rat anti-IgG2a, as secondary staining. Final staining was performed with streptavidin-APC, FITC-conjugated anti-rabbit T lymphocytes (or FITC-conjugated mouse IgG1 isotype control) and anti-human CD14 (or Pacific Blue-conjugated mouse IgG2a isotype control). Live lymphocytes were gated on 7-AAD- cells.

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