Altered intracellular ROS and caspase-1 and secreted IL-1β levels in rTgPrx exposed macrophages. (A & B) Cells were plated in sterile fluorescence plates overnight prior to stimulation and measurement of intracellular ROS. (C & D) Caspase-1 activity was measured in cells lysates by fluorescent enzyme assay 24 h after stimulation. (E & F) Mature IL-1β levels in supernatants were 24 determined by ELISA. *p < 0.05, **p < 0.01, ***p < 0.001, values represent a mean of triplicate wells ± SD; experiments were repeated three times with similar results.