STAT6 dependent and independent functions of rTgPrx. Cells were treated with the STAT6 inhibitor leflunomide for 30 min prior to stimulation or with vehicle control. (A) Cell lysates were used to determine the arginase activity by enzymatic assay. (B) IL-10 levels from supernatants weremeasured by ELISA. (C, D, & E) PCR for FIZZ (C), Ym1 (D), and Arg-1 (E) was carried out on RNA isolated from stimulated cells after 16 h. ***p < 0.001 values represent a mean of triplicate wells ± SD; experiments were repeated three times with similar results.