Activation of murine macrophages by rTgPRX. Cells were stimulated for 24 h under the indicated conditions and then analysed. (A) Cell lysates were tested for arginase activity by enzyme assay. (B) NO was measured in the supernatants from the same cultures as in (A). IL-12p40 (C) and IL-10 (D) were measured by ELISA in supernatants. PCR (E - G) was performed on cell 16 h after stimulation for FIZZ (E), Ym1 (F), and Arg-1 (G). rTgPrx at 80 μg/mL was used in panels (b), (c), and (d). p < 0.05 and ***p < 0.001, values represent a mean of triplicate wells ± SD; experiments were repeated three times with similar results.