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Table 1 Oligonucleotide sequences, amplicon lengths, GenBank accession numbers and standard curve evaluation for qPCR assays.

From: Transcription of reference genes used for quantitative RT-PCR in Atlantic salmon is affected by viral infection

Gene

Oligonucleotide sequence (5'-3')

Amplicon (bp)

GenBank acc. no

Slope

R2

Efficiency (E)*

EF1αB

TGCCCCTCCAGGATGTCTAC

57

BG933897

3.460

0.979

1.94

 

CACGGCCCACAGGTACTG

     
 

6FAM-AAATCGGCGGTATTGG-MGBNFQ

     

RPS20

GCAGACCTTATCCGTGGAGCTA

85

BG93667

3.055

0.979

2.12

 

TGGTGATGCGCAGAGTCTTG

     
 

6FAM-CCTCAAGGTGAAGGGA-MGBNFQ

     

β-actin

CCAAAGCCAACAGGGAGAAG

91

BG933897

3.173

0.996

2.06

 

AGGGACAACACTGCCTGGAT

     
 

6FAM-TGACCCAGATCATGTTT-MGBNFQ

     

18S rRNA

CCCCGTAATTGGAATGAGTACACTTT

98

AJ427629

3.092

0.978

2.10

 

ACGCTATTGGAGCTGGAATTACC

     
 

6FAM-CTTTAACGAGGATCCATTGG-MGBNFQ

     

SPDV

CCGGCCCTGAACCAGTT

107

AY604235

   
 

GTAGCCAAGTGGGAGAAAGCT

     
 

6FAM-CTGGCCACCACTTCGA-MGBNFQ

     
  1. * The amplification efficiency of each primer set was assessed according to the equation E = 10(1/-slope).
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Veterinary Research

ISSN: 1297-9716