Western blot analysis of PrPresin nervous tissue. Western blot analysis of PrPres in the nervous tissue sampled from the H-type BSE-challenged cattle (case 1, code 7749). 1, cranial cervical ganglion; 2, vagal nerve (cervical division); 3, vagal nerve (thoracic division); 4, phrenic nerve; 5, sympathetic trunk (thoracic division); 6, sympathetic trunk (lumbar division); 7, trigeminal ganglion; 8, adrenal gland (medulla); 9, facial nerve; 10, hypoglossal nerve; 11, celiac-mesenteric ganglion complex; 12, cauda equina; 13, suprascapular nerve. Each lane was loaded with 100 mg of tissue equivalent. Western blots were probed with mAb 6H4 to detect PrPres. Molecular mass standards (kDa) are indicated on the left.