Figure 6

Results of competitive multiplex qRT-PCR analysis to investigate temporal changes in the expression levels of a number of transcripts associated with tissue remodelling or inflammatory responses throughout the experiment trials summarised in Table 1. Data is shown for heat shock proteins HSPA8, HSPCA and STIP1; transcripts associated with pro-inflammatory responses CCL2, PLA2G2A and CF1, and matrix metalloproteinases and inhibitors MMP-13, MMP-23 and cystatin C, as indicated. Open circles represent data from naïve sheep; closed triangles represent data from immune ("previously infected") sheep. Significant difference between Nv and Im at same timepoint; *P ≤ 0.05, **P ≤ 0.005, ***P ≤ 0.005; Significant difference between timepoint and day 0 of same group (where available); ⁺P ≤ 0.05, ⁺⁺P ≤ 0.005, ⁺⁺⁺P ≤ 0.005 (Mann-Whitney U-test for non-parametric data).