Figure 5

Results of competitive multiplex qRT-PCR analysis to investigate temporal changes in the expression levels of mucous-cell associated transcripts; CLCA1 and ITLN s 1-3, as indicated, throughout the experiment trials summarised in Table 1. Open circles represent data from naïve sheep; closed triangles represent data from immune ("previously infected") sheep. Significant difference between Nv and Im at same timepoint; *P ≤ 0.05, **P ≤ 0.005, ***P ≤ 0.005; Significant difference between timepoint and day 0 of same group (where available); ⁺P ≤ 0.05, ⁺⁺P ≤ 0.005, ⁺⁺⁺P ≤ 0.005 (Mann-Whitney U-test for non-parametric data).