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Table 3 Proteomics-identified proteins for two N.risticii strains.

From: Neorickettsia risticii surface-exposed proteins: proteomics identification, recognition by naturally-infected horses, and strain variations

Locus ID Protein name Mol Mass (kDa) pIa % (query) peptide coverageb Signal peptidec
N. risticii Illinois T
NRI_0235 51-kDa antigen (P51) 54.9 8.44 49.2 (139) Yes (20-21)
NRI_0614 Heat shock protein 60 (GroEL) 58.1 5.23 43.2 (36) No
NRI_0841 Neorickettsia surface protein 3 (Nsp3) 25.7 5.96 12.0 (2) Yes (24-25)
NRI_0567 Conserved hypothetical protein 50.9 4.26 9.85 (2) No
PA-1
NRI_0235 P51 54.9 8.44 34.6 (41) Yes (20-21)
NRI_0614 GroEL 58.1 5.23 45.6 (36) No
NRI_0017 Heat shock protein 70 (DnaK) 68.4 5.18 2.20 (6) No
NRI_0132 ATP synthase F1, alpha subunit (AtpA) 55.9 5.29 2.75 (3) No
NRI_0872 Strain-specific surface antigen 3 (Ssa3) 41.9 6.01 2.36 or 4.72d (2) No
  1. aTheoretical isoelectric point of the given protein as predicted by ExPASy Compute pI/MW tool [64].
  2. bIndicates percentage coverage of proteins by all peptides. Numbers in parentheses are the number of peptide queries for each protein identified in the given band.
  3. cSignal peptide presence as determined by the Center for Biological Sequence Analysis SignalP v.3.0 [65]. Parentheses indicate amino acids between which cleavage is predicted to occur in the given protein.
  4. dThe peptide detected twice was within the repeated region of Ssa3, therefore the percentage coverage could be two different percentages.