Figure 4From: Neorickettsia risticii surface-exposed proteins: proteomics identification, recognition by naturally-infected horses, and strain variationsNsp2 amino acid sequence variations. (A) Amino acids different from N. risticii Illinois, including insertions and deletions are divided by the number of sequences plotted for each amino acid position (# aa diffs). The horizontal axis displays Nsp2 amino acid positions (aa position) including the signal peptide and all detected amino acid insertions (309 aa total). SP, signal peptide. E, external loop; and TM, transmembrane domain are based on the predicted secondary structure. The number of sequences available at each amino acid position on Nsp2 (# seqs) is shown below. (B) Dendrograms of Nsp2 from the full-length protein, including the signal peptide (12 sequences total) and (C) the predicted external loop 4 (55 aa, including all insertions; 19 sequences total) are shown with bootstrap values greater than 50.0% for 1000 replicates. *, bootstrap value of 90.0% or greater. Post-2000 sequences are shown in the shaded area. GenBank accession numbers of new sequences are listed in Table 1.Back to article page