PCR protocol | Primers (positions)* | Â | Amplification protocol | Reference |
---|---|---|---|---|
I | 1st PCR: | Â | Â | Â |
Product size*: | BTH-1F (365-385) | 5'cct gag aaa cgg cta cca cat ct | 94°C: 10 min, | [23] |
561 bp | BTH-1R (1031-1050) | 5' ttg cga cca tac tcc ccc ca | 40 cycles: 95°C: 30 s, 68°C: 1 min, 72°C: 1 min |  |
 |  |  | 72°C: 10 min |  |
 | nested PCR: |  |  |  |
 | GF2 (466-487) | 5' gtc ttg taa ttg gaa tga tgg | 94°C: 10 min | [19] |
 | GR2 (1006-1026) | 5' cca aag act ttg att tct ctc | 40 cycles: 95°C: 30 s, 60°C: 1 min, 72°C: 1 min |  |
 |  |  | 72°C: 10 min |  |
II | 1st PCR: | Â | Â | Â |
Product size*: | Babfor (959-977) | 5'gac tag gga ttg gag gtc | 94°C: 10 min | [24] |
576 bp | Babrev (1589-1610) | 5'gaa taa ttc acc gga tca ctc | 35 cycles: 95°C: 1 min, 53°C: 1.5 min, 72°C: 1.5 min |  |
 |  |  | 72°C: 10 min |  |
 | nested PCR |  |  |  |
 | BT2-F (1035-1055) | 5'gga gta tgg tcg caa gtc tg | 94°C: 10 min | [23] |
 | Babrev |  | 35 cycles: 95°C: 1 min, 53°C: 1.5 min, 72°C: 1.5 min |  |
 |  |  | 72°C: 10 min |  |