TLRs in tissues of H9N2-infected chickens. Induction of TLRs using total RNA (Figure 2) was quantified using primers specific for chicken TLRs and SYBR Green-based real-time polymerase chain reaction. Data represent the mean ± standard error of five chickens. Panels A, B, and C depict results from trachea, lung, and intestinal tissue. Statistical analysis was performed by the comparison of data of uninfected chickens. [*P < 0.05], [**P < 0.001].