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Table 3 Transcription profile of genes during a primary infection with O.ostertagi

From: Infection with the gastrointestinal nematode Ostertagia ostertagi in cattle affects mucus biosynthesis in the abomasum

Gene

  

Infectionb

 

6 dpi

9 dpi

24 dpi

60 dpe

Mucins

    

Membrane bound

    

MUC1

2.07 ± 0.3*

2.93 ± 0.3*

3.29 ± 0.3*

2.75 ± 0.2*

MUC20

2.78 ± 0.5*

1.50 ± 0.2*

3.99 ± 0.6*

3.28 ± 0.6*

Secreted gel forming

    

MUC2

n.dc

n.d

n.d

n.d

MUC5AC

4.08 ± 1.1*

2.25 ± 0.5*

1.50 ± 0.2*

1.56 ± 0.7*

MUC5B

n.d

n.d

n.d

n.d

MUC6

3.09 ± 1.0*

2.80 ± 0.4*

4.39 ± 1.4*

4.52 ± 0.5*

Trefoil Factors

    

TFF1

3.89 ± 1.5*

3.32 ± 0.8*

2.57 ± 0.2*

3.90 ± 0.5*

TFF2

2.50 ± 1.1*

4.12 ± 2.4*

2.22 ± 0.8*

3.81 ± 1.4*

TFF3

2.59 ± 0.8*

18.57 ± 11.7*

58.57 ± 13.2*

49.28 ± 16.7*

Glycosyltransferases

    

Core structure

    

GCNT3

2.93 ± 0.6*

3.73 ± 0.3*

5.90 ± 1.0*

4.29 ± 0.6*

GCNT4

2.92 ± 0.3*

2.33 ± 0.2*

3.02 ± 0.2*

3.14 ± 0.3*

C1GALT1

3.06 ± 0.6*

2.09 ± 0.2*

2.22 ± 0.5*

2.35 ± 0.2*

Backbone and terminal

    

GCNT2

2.87 ± 0.8*

1.60 ± 0.2

12.44 ± 4.2*

6.74 ± 1.0*

A4GNT

3.32 ± 0.3*

2.03 ± 0.3

3.12 ± 0.8*

2.75 ± 0.1*

B3GNT3

4.51 ± 1.1*

3.11 ± 0.6*

2.57 ± 0.8*

4.61 ± 0.4*

Sulfotransferases

    

H3ST1

1.14 ± 0.2*

2.10 ± 0.6*

4.52 ± 2.6*

2.35 ± 0.5*

H3ST2

n.d

n.d

n.d

n.d

GAL3ST1

n.d

n.d

n.d

n.d

Sialyltransferases

    

ST3GAL4

1.60 ± 0.1*

1.20 ± 0.2*

1.89 ± 0.3*

1.15 ± 0.1*

Fucosyltransferases

    

FUT1

1.94 ± 0.5*

1.27 ± 0.2*

0.77 ± 0.1*

1.33 ± 0.1*

FUT2

2.35 ± .4*

2.56 ± 0.8*

3.13 ± 0.4*

3.15 ± 0.2*

FUT4

2.77 ± 0.5*

2.89 ± 1.0*

3.09 ± 0.5*

3.13 ± 0.6*

FUT10

1.65 ± 0.2*

1.47 ± 0.2*

1.46 ± 0.2*

1.58 ± 0.1*

Disulphide isomerase

    

AGR2

3.41 ± 0.6*

3.44 ± 0.4*

5.82 ± 0.9*

5.24 ± 0.8*

PDIA3

3.16 ± 0.3*

2.60 ± 0.4*

4.12 ± 1.0*

4.46 ± 0.6*

PDIA4

2.58 ± 0.2*

3.51 ± 0.8*

6.95 ± 0.9*

5.37 ± 0.9*

  1. a
  2. amean ± error standard of fold changes in infected animals compared to control (0 dpi).
  3. bdpi: days post-infection dpe: days post-exposure.
  4. cn.d.: not determined, too low in the sample.
  5. *p value ≤ 0.05 control 0 dpi vs infected animals (6, 9, 24 dpi; 60 dpe).