Table 1 Primers used to produce PCV-2 DNA clones
From: Modification of PCV-2 virulence by substitution of the genogroup motif of the capsid protein
Primer name | Primer sequence 5'-3' | Primer purpose |
|---|---|---|
PCV-2a short forward | AAGTATTACCAGCGCACTTC | Amplification |
PCV-2a short reverse | ACCATTACGAAGTGATAAAA | Amplification |
PCV-2a long forward | CCATGCCCTGAATTTCCATA | Amplification |
PCV-2a long reverse | CCGTGGATTGTTCTGTAGCA | Amplification |
PCV-2b forward | ACAACGGAGTGACCTGTCTA | Amplification |
PCV-2b reverse | ACCATTACGAAGTGATAAAA | Amplification |
PCV-2a/(SNPISI) forward | CCCCGGGAGGGGGGAG CAACCC AATCTCTATACCCTTT | Mutation |
PCV-2a/(SNPISI) reverse | AAAGGGTATAGAGATTGG GTTGC TCCCCCCTCCCGGGG | Mutation |
PCV-2a/motif 2b forward | GGGAGGGGGGAGCAACCCAAGG TCTG TACCCTTTGAATAC | Mutation |
PCV-2a/motif 2b reverse | GTATTCAAAGGGTAC AGACC TTGGGTTGCTCCCCCCTCCC | Mutation |
PCV-2b/(TNKRSV) forward | CTTCCCCCAGGAGGGGGCA CG AACAAG CGCTCTGTGCCCTTTGAA | Mutation |
PCV-2b/(TNKRSV) reverse | TTCAAAGGGCACAGAGCGCTT GTTC GT GCCCCCTCCTGGGGGAAG | Mutation |
PCV-2b/motif 2a forward | CCCAGGAGGGGGCACGAACAAGAT CTCTA TA CCCTTTGAATACTACAGAATAA | Mutation |
PCV-2b/motif 2a reverse | TTATTCTGTAGTATTCAAAGGGT AT AGAGAT CTTGTTCGTGCCCCCTCCTGGG | Mutation |