Phylogenetic tree showing the equine picobirnavirus strain (Genogroup I PBV/Horse/India/BG-Eq-3/2010) with cognate stretch of hitherto reported human, porcine, bovine, canine, murine and serpentine genogroup I PBV strains, based on partial amino acid sequence (56 amino acids (aa)) of genomic segment 2. The phylogenetic tree was constructed by the neighbor-joining method using the MEGA software (Version 4.1). Phylogenetic distances were measured by the Kimura two-parameter model, and the tree was statistically supported by bootstrapping with 1000 replicates. The genogroup I equine PBV strain BG-Eq-3 is denoted with a ● symbol. The tree was rooted with cognate stretch of gene segment 2 of genogroup II prototype strain Hu/4-GA-91 (USA) defined as the outgroup strain. Abbreviations: Hu, Human; Po, Porcine; Bo, Bovine; Eq, Equine; ARG, Argentina; BRA, Brazil; Hun, Hungary; Thai, Thailand; USA, United States of America; Ven, Venezuela.