Detection of bovine and human recombinant IL-8 using the human-specific IL-8 ELISA kit. For each data point, the concentration of bovine IL-8 (-■-) was 100 times that of the human IL-8 (-▲-). The concentration of the bovine IL-8 is indicated on the x-axis. The error bars indicate S.D. (n = 3). A450 without added cytokine was 0.08 ±S.D. 0.0016 (n = 6).