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Table 4 In situ probes used for visualisation of Francisella spp. in aquatic animals.

From: Francisella infections in farmed and wild aquatic organisms

Fish type Bacterial species Probe type¤ Primers or probes (5' - 3') Reference
Cichlids Francisella spp. PCR product (286bp) FLB16S180f
gcg-gat-taa-agg-tgg-cct-ttg-c
Hsieh et al. [42]
    FLB16S465r
cct-gca-agc-tat-taa-ctc-aca-gg
 
Tilapia Francisella sp.* PCR product (1113bp) F5
cct-ttt-tga-gtt-tcg-ctc-c
Hsieh et al. [2]#
    F11
tac-cag-ttg-gaa-acg-act-gt
 
Three-lined grunt Francisella noatunensis subsp. orientalis Antisense oligo- Isaki-DIG-80r
ctc-gtc-agc-atc-cga-aga-cct-gtt-a
Kamaishi et al. [3]
    Isaki-DIG-200r
ggc-agc-gca-aag-gcc-acc-ttt-aat-ccg-cag-ata-t
 
Abalone Francisella sp. Antisense oligo- Megai-110r
ccg-cca-ctc-gtc-agc-aag-aag-caa-gct-tct-cct-gtt-acc-gtt-cga-ctt-gc
Kamaishi et al. [8]
    Megai-230r
cta-acg-cag-gct-cat-cca-tct-gcg-gca-gca-caa-agg-cca-cct-tta-atc-ctc-aga-tag-tat
 
    Megai-870r
gag-tac-tta-acg-cgt-tag-cta-cgc-cac-tag-atc-ctt-tac-acc-gaa-tcc-aac-agc-tag-tac
 
  1. ¤ all Dioxigenin marked; * Presumptively F. noatunensis subsp. orientalis.
  2. # primers originally published by Forsman et al. [64].