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Table 1 Neospora caninum isolates included in the in vitro assays.

From: In vitro invasion efficiency and intracellular proliferation rate comprise virulence-related phenotypic traits of Neospora caninum

Isolate* Host origin** Geographical origin& Passages number§ Neonatal morbidity
Neonatal mortality
transmission rate
Nc-Spain 1Ha 2-day-old healthy calf Madrid 12-18 0L 5L 5L
Nc-Spain 2H 2-day-old healthy calf Zaragoza 7-13 46.1L 20.4L 61.3L
Nc-Spain 3Hb 52-day-old healthy calf Navarra1 19-25 10.6L 7.7L 89H
Nc-Spain 4Hb 22-day-old healthy calf Navarra1 12-18 100H 100H 97.3H
Nc-Spain 5H 14-day-old healthy calf León 12-18 98.6H 96H 100H
Nc-Spain 6 30-day-old healthy calf País Vasco 20-26 34.5L 29.8L 57.6L
Nc-Spain 7 57-day-old healthy calf Navarra2 17-23 98.3H 95H 79.1L
Nc-Spain 8 2-day-old healthy calf Navarra1 8-14 4.7L 1.1L 56.4L
Nc-Spain 9 7-day-old healthy calf Navarra2 9-15 39L 32.5L 52.6L
Nc-Spain 10a 2-day-old affected calf? Madrid 21-27 25.5L 17.9L 65.5L
Nc-Liverpool 4-week-old affected dog UK 12R-19R@ 100H 100H 95.6H
  1. Summary of name, genetic characterisation, host and geographical origin, passage number in cell culture, and their pathogenicity in a BALB/c pregnant mouse model determined in previous studies [14, 16].
  2. * Nc-Liv and Spanish isolates were genetically characterised by microsatellite analysis. Letters in superscript (a) and (b) indicate isolates with identical microsatellite profiles [4, 5, 14].
  3. **All Spanish isolates were obtained from asymptomatic calves from different cattle. Nc-Spain 10 was isolated from an affected calf, but its clinical signs could not be attributed to Neospora infection [5, 14]. Nc-Liv was isolated from a clinically affected dog [50].
  4. &The geographical origin of Spanish isolates is indicated by province. Calves from Navarra originated from two dairy herds. Numbers in superscript (1) and (2) identify the dairy herd. Nc-Liverpool was isolated in the United Kingdom.
  5. §The total number of cell culture passages of the N. caninum isolates included in these in vitro assays. (@) marks the total passages after re-isolation of Nc-Liv in cell culture from BALB/c nu/nu mice.
  6. #The percentages of neonatal morbidity and neonatal mortality and vertical transmission rates were determined in previous studies using a pregnant BALB/c mouse model [14, 16]. L, H Isolates were categorised into lowly/moderately pathogenic (L) or highly pathogenic (H) groups according to the significant differences found in neonatal morbidity and mortality. Identical superscript letters denote highly (H) and lowly/moderately transmissible (L) isolates.