Isolate* | Host origin** | Geographical origin& | Passages number§ | Neonatal morbidity (%)# | Neonatal mortality (%)# | Vertical transmission rate (%)# |
|---|
Nc-Spain 1Ha | 2-day-old healthy calf | Madrid | 12-18 | 0L | 5L | 5L |
Nc-Spain 2H | 2-day-old healthy calf | Zaragoza | 7-13 | 46.1L | 20.4L | 61.3L |
Nc-Spain 3Hb | 52-day-old healthy calf | Navarra1 | 19-25 | 10.6L | 7.7L | 89H |
Nc-Spain 4Hb | 22-day-old healthy calf | Navarra1 | 12-18 | 100H | 100H | 97.3H |
Nc-Spain 5H | 14-day-old healthy calf | León | 12-18 | 98.6H | 96H | 100H |
Nc-Spain 6 | 30-day-old healthy calf | País Vasco | 20-26 | 34.5L | 29.8L | 57.6L |
Nc-Spain 7 | 57-day-old healthy calf | Navarra2 | 17-23 | 98.3H | 95H | 79.1L |
Nc-Spain 8 | 2-day-old healthy calf | Navarra1 | 8-14 | 4.7L | 1.1L | 56.4L |
Nc-Spain 9 | 7-day-old healthy calf | Navarra2 | 9-15 | 39L | 32.5L | 52.6L |
Nc-Spain 10a | 2-day-old affected calf? | Madrid | 21-27 | 25.5L | 17.9L | 65.5L |
Nc-Liverpool | 4-week-old affected dog | UK | 12R-19R@ | 100H | 100H | 95.6H |
- Summary of name, genetic characterisation, host and geographical origin, passage number in cell culture, and their pathogenicity in a BALB/c pregnant mouse model determined in previous studies [14, 16].
- * Nc-Liv and Spanish isolates were genetically characterised by microsatellite analysis. Letters in superscript (a) and (b) indicate isolates with identical microsatellite profiles [4, 5, 14].
- **All Spanish isolates were obtained from asymptomatic calves from different cattle. Nc-Spain 10 was isolated from an affected calf, but its clinical signs could not be attributed to Neospora infection [5, 14]. Nc-Liv was isolated from a clinically affected dog [50].
- &The geographical origin of Spanish isolates is indicated by province. Calves from Navarra originated from two dairy herds. Numbers in superscript (1) and (2) identify the dairy herd. Nc-Liverpool was isolated in the United Kingdom.
- §The total number of cell culture passages of the N. caninum isolates included in these in vitro assays. (@) marks the total passages after re-isolation of Nc-Liv in cell culture from BALB/c nu/nu mice.
- #The percentages of neonatal morbidity and neonatal mortality and vertical transmission rates were determined in previous studies using a pregnant BALB/c mouse model [14, 16]. L, H Isolates were categorised into lowly/moderately pathogenic (L) or highly pathogenic (H) groups according to the significant differences found in neonatal morbidity and mortality. Identical superscript letters denote highly (H) and lowly/moderately transmissible (L) isolates.
