Figure 2

Plot graphs representing the proliferation kinetics over time, as assessed by real-time PCR (A), and the linear regression of the average numbers of tachyzoites (ln-transformed) determined by real-time PCR against the time of the exponential phase (B) for each isolate included in this study (see graph legend). The average number of tachyzoites for each time in plot graphs A and B is representative of all of the individual experiments with an R² > 0.95, and the error bars indicate the SD. Line slopes in plot graph B define the proliferation rate (μ) and doubling time (Td, Ln 2/μ). For all isolates, R² > 0.98.