The liver granuloma reaction after L. infantum infection. (A) The histology of the liver and cellular infiltrates in infected mice at 28 days pi (×40). Compare (A) with the detail in the lower right corner of the image (non-infected mice showing normal histological appearance, ×40). Liver granuloma evolution: (B) immature granulomas. Early parasitization of KCs (arrows) with initial cell recruitment (×400), at 14 days pi. (C) Mature granuloma assembly at infected KCs, resulting in the attraction of lymphocytes and monocytes, at 28 days pi (×400). (D) Mature-sterile granuloma, free of amastigotes, at 56 days pi (×200).