Figure 5
From: NKp46 defines ovine cells that have characteristics corresponding to NK cells
Cultured Ovine NKp46+ cells exhibited cytotoxic activity. Cytotoxicity of NKp46+ cultures from five animals as determined in 4 h-111In release assays. Lysis of the xenogenic NK-sensitive cells lines K562 and YAC1 exhibited inter-animal variation, with lysis ranging from approximately 3-22% at effector to target ratios of 8:1. Direct lysis of P815 cells (FcγR+) was low (6-14% at effector to target ratios of 8:1), but in redirected cytotoxicity assays pre-incubation with anti-NKp46 antibody (EC1.1) dramatically increased cytotoxic activity (62-81% at effector to target ratios of 8:1) of all NKp46+ cultures. Pre-incubation with an isotype control antibody did not elevate cytotoxicity above that seen in the absence of antibody.