Figure 3
From: NKp46 defines ovine cells that have characteristics corresponding to NK cells
Analysis of the phenotype of ovine NKp46+ populations. (A)Two-colour flow cytometry analysis of ovine PBMC co-stained with EC1.1 and a selection of monoclonal antibodies against CD2, CD4, CD8, CD11b, CD16, WC1, IgM and MHCII. WC1 and IgM are surface molecules expressed by subsets of γδ T-cells and B-cells respectively. (B) Perforin expression by NKp46+ PBMC. Cells expressing NKp46 were stained with anti-perforin monoclonal antibody (solid line) or an isotype control (broken line). (C) Two-colour flow cytometry analysis of mesenteric and pre-scapular lymph nodes and the spleen cell populations co-stained with EC1.1 and monoclonal antibodies against CD2 and CD8. The results shown are representative of the results obtained from a minimum of five animals.