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Table 2 Proteins identified in this study.

From: Staphylococcus aureus seroproteomes discriminate ruminant isolates causing mild or severe mastitis

Description1 Spots2 O11 CDS3 O46 CDS3 ED133 CDS3 pI4 Mass (Da)5 Score6 Cov.7 #pep.8 EmPAI9 Comp.10 Loc.11 Immun.12
Metabolism              
Energy production and conversion              
formate acetyltransferase 81, 83, 82 011_0041 046_0511 SAOV_0163 5,31 84808 1301,60 34,31 22 1,57 CW C  
ldh L-lactate dehydrogenase 82, 99, 81, 83, 95 011_0136 046_0198 SAOV_2646c 4,80 34389 1749,37 63,64 26 21,75 CW C [82]
L-LACTATE DEHYDROGENASE (O11) 64 011_0021 046_0531 SAOV_0178 5,00 34548 745,83 50,79 14 2,59 S C [82]
bifunctional acetaldehyde-CoA/alcohol dehydrogenase 79 011_0796 046_0709 SAOV_0095 5,68 94945 2090,64 45,45 33 2,26 CW C  
D-3-phosphoglycerate dehydrogenase 100 011_0585 046_1131 SAOV_2344 5,32 34652 717,85 43,53 11 1,72 CW C  
atpD F0F1 ATP synthase subunit beta 62, 90 011_2064 046_0898 SAOV_2144c 4,68 51368 631,03 30,21 10 0,98 S,CW C [83]
Nucleotide transport and metabolism              
adk adenylate kinase 9 011_0510 046_1206 SAOV_2266c 4,80 23375 531,72 42,38 9 2,80 S C [84]
deoD purine nucleoside phosphorylase 9 011_1051 046_0577 SAOV_2178 4,85 25892 470,94 53,39 8 1,97 S C [4]
guaB inositol-monophosphate dehydrogenase 102, 129 011_0077 046_0960 SAOV_0412 5,61 52818 1237,95 54,30 20 3,52 CW,T C [4]
Carbohydrate transport and metabolism              
gpmA 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase 131 011_1952 046_0264 SAOV_2463c 5,23 26663 271.65 30.26 5 0.80 S,T C  
eno enolase 2-phosphoglycerate dehydratase 62, 88, 90, 96, 137 011_2336 046_2241 SAOV_0818 4,55 47088 1868,52 63,59 25 7,09 S,CW,T C [4, 52, 8587]
fda fructose-1,6-bisphosphate aldolase 107, 94, 72, 91, 95 011_0131 046_0202 SAOV_2650 5,06 32878 852,09 46,62 13 2,48 CW,S C [8588]
gap glyceraldehyde-3-phosphate dehydrogenase 99, 57, 81, 82, 83 011_2340 046_2237 SAOV_0814 4,89 36258 940,06 46,13 13 3,04 CW,S C [82, 86, 87, 89, 90]
putative translaldolase 9 011_1872 046_2451 SAOV_1765 4,72 25689 554,11 46,84 10 3,32 S C  
tpiA triosephosphate isomerase 9 011_2338 046_2239 SAOV_0816 4,81 27271 1396,16 76,28 21 16,78 S C [4, 91, 92]
atl autolysin 23, 31 011_1991 046_0320 SAOV_0999c 9,59 136983 2567,68 42,14 42 1,87 S S [52, 76, 93]
pyk pyruvate kinase 105 011_1282 046_0580 SAOV_1685 5,23 63067 271,45 11,62 5 0,29 CW C [94]
Lipid metabolism              
fabZ 3R-hydroxymyristoyl ACP dehydratase 126 011_2356 046_1839 SAOV_2140c 5,71 16071 86,65 13,70 2 0,47 T C  
acetoin reductase 106 011_1332 046_1393 SAOV_0074 5,04 27199 566,84 40,31 7 1,82 CW C  
Amino acid transport and metabolism              
dipeptidase PepV 62 011_1904 046_1329 SAOV_1737 4,56 52762 235.33 10.66 4 0,27 S C  
CYSTEINE SYNTHASE (O11) 64 011_1382 046_2402 SAOV_0535 5,38 32955 266.80 28.71 5 0,61 S C  
Information storage and processing              
Translation, ribosomal structure and biogenesis              
fus elongation factor G 95 011_0401 046_1769 SAOV_0582 4,80 76564 2173,77 68,11 30 4,11 CW C [3, 90]
prs 50S ribosomal protein L25/general stress protein Ctc 94 011_1370 046_2414 SAOV_0523 4,39 23773 384,17 34,56 6 2,26 CW,S C [95]
rplC 50S ribosomal protein L3 131 011_0531 046_1185 SAOV_2287c 9,72 22648 482,78 42,11 9 2,95 T C [95]
rpsA 30S ribosomal protein S1 137, 62, 121, 171 011_2142 046_1743 SAOV_1482 4,51 43252 1555,46 71,36 23 6,25 S,CW,T C [39, 95]
rpsD 30S ribosomal protein S4 139 011_1260 046_1365 SAOV_1706 10,02 22999 428,22 40,50 8 1,96 T C [95]
rplB 50S ribosomal protein L2 91 011_0528 046_1188 SAOV_2284c 10,77 30136 239,93 25,27 5 0,69 CW C [95]
tsf elongation factor Ts 100, 64 011_0909 046_0755 SAOV_1259 5,05 32474 436,02 31,06 7 0,97 CW,S C [4, 86, 93, 96]
tuf elongation factor Tu 90, 88, 96, 10, 122, 137, 149, 81, 82, 83, 95 011_0402 046_1768 SAOV_0583 4,74 43077 2665,48 84,26 38 52,14 S,CW,T C [3, 52, 82, 85, 86, 90, 93, 96]
yfiA ribosomal subunit interface protein 106 011_2483 046_1255 SAOV_0789 5,25 21511 376,75 35,33 6 1,76 CW C  
aspS aspartyl-tRNA synthetase 63 011_2454 046_2303 SAOV_1627 4,99 66584 233,90 7,65 4 0,21 S C  
alaS alanyl-tRNA synthetase 82 011_2466 046_2291 SAOV_1616 5,00 98604 442,31 11,74 8 0,30 O C [97]
Transcription              
nusA transcription elongation factor NusA 62 011_0919 046_0765 SAOV_1268 4,60 43701 247,74 11,00 4 0,34 S C  
DNA replication, recombination and repair              
dnaN DNA polymerase III subunit beta 90 011_1166 046_1471 SAOV_0002 4,66 41888 706,28 45,62 11 1,30 CW C  
nuc staphylococcal thermonuclease precursor 151, 108, 5, 153, 206, 207, 217 011_2070 046_2528 SAOV_0832 9,20 25089 967,00 50,00 20 14,46 S,CW PSE [98]
ruvA Holliday junction DNA helicase 66 011_2442 046_2315 SAOV_1639 5,77 22249 137,72 17,00 3 0,52 S C  
Posttranslational modification, protein turnover, chaperones              
ahpC alkyl hydroperoxide reductase subunit C 67, 82, 83, 99, 95 011_0085 046_0968 SAOV_0404c 4,88 20963 667,36 56,08 11 4,95 S,CW C [93]
AhpF ALKYL HYDROPEROXIDE REDUCTASE SUBUNIT F (O11) 97, 86 011_0086 046_0969 SAOV_0403c 4,68 54655 2242,14 67,06 34 9,91 CW C  
dnaK chaperone protein 96, 173, 137 011_2230 046_2216 SAOV_1580 4,63 46021 2907,83 80,29 43 24,66 S,CW,T C [90, 96, 99]
peptidyl-prolyl cis-isomerase 212, 1, 68, 91 011_2089 046_2477 SAOV_1837 9,01 35602 574,32 31,56 11 1,66 S,CW PSE  
tig trigger factor 105 011_1304 046_0602 SAOV_1664 4,34 48565 1061,87 61,43 17 2,25 CW C [94]
TrxB THIOREDOXIN REDUCTASE (O11) 64 011_2580 046_2228 SAOV_0801 5,21 33595 681,95 33,76 11 1,81 S C [93]
Cellular processes              
Cell envelope biogenesis, outer membrane              
IsaA IMMUNODOMINANT ANTIGEN A (O46) 185, 8, 161, 186, 9, 164, 189 011_0168 046_0166 SAOV_2614c 5,91 24219 1516,56 59,23 22 40,99 S S [3, 75]
isdA iron-regulated cell wall-anchored protein 31, 27, 73, 74, 75, 83, 118, 79, 81, 82, 95 011_1476 046_1296 SAOV_1125c 8,69 70445 2288,12 57,87 38 6,04 S,CW PSE [100102]
isdB cell surface transferrin-binding protein 212, 211, 210, 208, 193, 156, 138, 110, 70, 86, 131, 156, 193 011_1477 046_1295 SAOV_1126c 9,54 39197 886,30 45,48 15 4,45 S,CW,T PSE [54, 100, 103]
IRON-REGULATED SURFACE DETERMINANT PROTEIN H (O11) 55, 31 011_1248 046_1353 SAOV_1717 5,05 100650 2209,72 42,11 37 2,58 S PSE [75]
IsdD iron-regulated protein 15, 16 011_1480 046_1292 SAOV_1128 8,51 41357 278,05 15,36 5 0,47 S PSE  
Cell motility and secretion              
N-acetylmuramoyl-L-alanine amidase 52, 51, 187 011_1090 046_1546 SAOV_2693 5,87 69226 3097,93 71,57 43 16,52 S S [57, 75, 76]
Inorganic ion transport and metabolism              
nasE assimilatory nitrite reductase 10 011_1932 046_0245 SAOV_2445c 4,95 11430 126,05 23,08 2 0,70 S C  
mntC Manganese/iron transport system substrate-binding protein 94 011_2274 046_0062 SAOV_0666c 8,68 34719 1183,08 51,46 20 10,68 S,CW PSE  
sirA iron-regulated lipoprotein 135, 64 011_1345 046_1405 SAOV_0062 9,20 36735 609,79 35,45 11 1,58 S,T PSE [104]
fhuD2 ferrichrome-binding protein 91 011_0566 046_1150 SAOV_2323c 9,16 33990 406,75 30,13 8 1,10 CW PSE [105]
ferrichrome ABC transporter lipoprotein 91 011_1857 046_1674 SAOV_2224c 9,44 36751 600,45 38,41 11 1,57 CW PSE  
Signal transduction mechanisms              
SA1540 GAF domain-containing protein 10 011_1261 046_1366 SAOV_1705 5,09 17042 139,34 22,73 3 0,72 S C  
Universal stress response protein 7, 123 011_1269 046_1374 SAOV_1697 5,60 18463 973,68 78,31 12 19,34 S,CW C [106]
Toxins and haemolysins              
beta-hemolysin 15, 19, 205 011_1750 046_2394 SAOV_2040 8,75 37386 1308,79 61,03 20 4,92 S S [57, 107]
hla alpha-hemolysin precursor 13, 1, 15, 19, 21, 68, 72, 107, 146, 153 011_1514 046_1259 SAOV_1161c 8,87 36329 2238,72 76,71 34 44,90 S,CW,T S [93, 107]
hlgC gamma-hemolysin component C 68, 1 011_1956 046_0268 SAOV_2469 9,29 35562 765,07 31,43 12 1,90 S,CW S [57]
Virulence/defence mechanisms              
Sbi IGG-BINDING PROTEIN SBI (O11) 216, 217, 212 011_1954 046_0266 SAOV_2466 9,38 49998 984,16 41,74 18 2,80 S S [76]
SspB CYSTEINE PROTEASE PRECURSOR (O11) 58, 57, 64, 182, 215 011_2154 046_0327 SAOV_0993c 5,45 42714 2027,86 65,52 30 11,52 S S [4]
SplF serine proteinase 4 011_0672 046_2496 SAOV_1795 9,36 25638 255,88 23,01 5 0,84 S S [108]
lukD leukotoxin D subunit 1 011_0685 046_2484 SAOV_1812 9,14 36936 365,37 22,02 7 0,98 S S [109]
lukE leukotoxin E subunit 1, 68 011_0686 046_2483 SAOV_1813c 9,38 34126 451,11 17,65 7 1,77 S,CW S [109]
leukocidin chain lukM precursor 68, 1, 120, 145, 177, 194, 201, 202, 94 011_1215 046_2777 SAOV_1909 9,41 35054 2504,69 71,43 35 29,80 S,CW,T S [58, 110]
leukocidin F subunit 16, 70 011_1752 046_1972 SAOV_2041 8,29 38639 1154,65 45,27 19 11,64 S,CW S [58, 110]
leukocidin S subunit 199, 5, 70, 108, 109, 197, 200, 211 011_1753 046_1973 SAOV_2042 9,38 40379 1399,91 55,56 23 6,70 S,CW S [58, 110]
Panton-Valentine leukocidin LukF-PV chain precursor 1, 68, 70, 15, 31, 119, 195, 203, 204, 10, 91 011_1216 046_2776 SAOV_1908 9,16 36496 965,38 50,31 15 3,37 S,CW S [58, 110]
plc 1-phosphatidylinositol phosphodiesterase 11, 12, 19 011_1424 046_1419 SAOV_0049 7,12 37030 2759,20 71,95 44 84,00 S S [4]
Aur ZINC METALLOPROTEINASE AUREOLYSIN (O11) 220, 63 011_1083 046_1553 SAOV_2686c 4,98 54947 921,93 47,39 17 1,68 S C [76, 111]
Opp1A OLIGOPEPTIDE TRANSPORTER SUBSTRATE BINDING PROTEIN (O11) 86 011_2424 046_2102 SAOV_2517c 8,33 59224 1398,01 47,53 24 3,28 CW PSE  
SspA GLUTAMYL ENDOPEPTIDASE SERINE PROTEASE (O11) 56, 184, 214 011_2155 046_0325 SAOV_0994c 4,68 32250 1575,55 69,26 24 24,18 S C [57]
SECRETED VON WILLEBRAND FACTOR-BINDING PROTEIN (O11) 22 011_2679 046_0987 SAOV_2051c 8,39 57935 1122,89 36,67 17 1,70 S S  
epidermal cell differentiation inhibitor B 208, 209, 193, 156, 4 011_0489 046_2741   9,51 27969 1385,18 69,32 22 22,31 S S  
Miscellaneous              
adhA alcohol dehydrogenase 100 011_1554 046_0086 SAOV_0640 5,34 36025 1167,82 66,37 16 4,30 CW C [87, 93]
exported secretory antigen precursor 67 011_0584 046_1132 SAOV_2343 5,77 17388 397,17 33,13 5 1,43 S S  
lip triacylglycerol lipase precursor 31, 27 011_1119 046_1518 SAOV_2721c 8,13 76637 1723,17 50,95 29 2,36 S S [93]
mqo malate:quinone oxidoreductase 128 011_0130 046_0203 SAOV_2651c 6,12 55964 1047,99 46,79 17 2,50 T C  
putative staphylococcal enterotoxin 151 011_0097 046_0981 SAOV_0394 9,54 23311 173,20 18,72 4 0,71 S S  
Unknown              
HYPOTHETICAL PROTEIN (O11) 220 011_0736 046_1969 SAOV_0454 4,89 56229 823,11 39,40 14 1,21 S S  
hypothetical protein (Similar to truncated map-w protein (91%)) 88, 10, 62 011_1749 046_2393   9,91 53686 899,64 34,52 18 2,09 S,CW S [112]
hypothetical protein (Similar to beta-lactamase (84%)) 10 011_0679 046_2490   6,84 20800 726,52 47,15 10 7,09 S S  
HYPOTHETICAL PROTEIN (O46) 157, 160, 175, 189, 193 011_0490 046_2740   8,88 30854 2343,27 74,29 41 65,07 S S  
hypothetical protein (Similar to probable glutamyl-endopeptidase (76%)) 66 011_0488 046_2742   5,44 20552 741,93 47,62 12 5,11 S C  
SA0570 HYPOTHETICAL PROTEIN (O46) 219,154, 127 011_2290 046_0078 SAOV_0649 9,17 18554 815,59 69,05 13 15,93 S,CW S  
SA0663 hypothetical protein 10, 164, 94 011_2561 046_2636 SAOV_0742c 9,15 16047 457,11 35,62 8 3,63 S PSE  
SA0914 hypothetical protein 6 011_2000 046_0311 SAOV_1008c 6,55 11338 522,48 57,14 8 13,10 S S  
pathogenicity island protein 55 011_2741 046_0985 SAOV_0429 9,55 12071 204,61 19,64 3 1,12 S S  
SA1607 hypothetical protein 91 011_1867 046_2456 SAOV_1770 6,04 34973 232,41 16,56 4 0,43 CW S  
SA1402 hypothetical protein 91 011_2223 046_2209 SAOV_1573 5,60 35160 448,93 28,27 6 0,72 CW S  
  1. Gene products of the accessory seroproteome are indicated in bold capital letters, (O11) indicates O11-specific proteins, (O46) indicates O46-specific proteins.
  2. All the other proteins belong to the core seroproteome
  3. 1: Proteins are classified in Gene Ontology functional classes. Names are given according to annotation of available S. aureus sequence genomes.
  4. 2: Spot number (see figures)
  5. 3: Coding sequence numbers corresponding to the identified proteins in S. aureus O11, S. aureus O46, and ED133, respectively.
  6. 4: Theoretical isoelectric point as determined from the predicted protein sequence
  7. 5: Theoretical Mass as determined from the predicted protein sequence
  8. 6: Mascot standard score
  9. 7: % of the protein sequence covered by the peptides identified
  10. 8: number of peptides identified
  11. 9: exponentially modified protein abundance index
  12. 10: Subproteome compartment where the spot was identified. CW, cell wall; S, supernatant; T, total fraction
  13. 11: predicted protein localization. C, cytoplasmic; S, secreted; PSE, predicted surface exposed
  14. 12: reported as immunogen elsewhere
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