Figure 2
From: Effects of polymorphisms in ovine and caprine prion protein alleles on cell-free conversion
Dominant inhibition of the cell-free conversion by alleles associated with low susceptibility. (A) PrPres fragments generated from 136A (lane 1), a 1:1 mxiture of 136A with BSA (lanes 2-3), a 1:1 mixture of 136A with 171R (lanes 4-5), a 1:1 mixture of 136A with 154H (lanes 6-7). PrPres fragments derived from IHNRRQ (lane 8), a 1:1 mixture of IHNRRQ with 146S (lanes 9-10), a 1:1 mixture of IHNRRQ with 146D (lanes 11-12) and a 1:1 mixture of IHNRRQ with 222K (lanes 13-14). Detection was carried out using mab P4. Arrow indicates PrPres fragments. (B) Mean relative conversion efficiencies (± SEM) for each set of at least four conversion reactions. Relative conversion rates were calculated in relation to the ovine 136A reference allele and the caprine IHNRRQ reference allele. The differences were analyzed by unpaired student t-test. *: p < 0.05; **: p < 0.01; ***: p < 0.001. ns: not significant.