Figure 1
From: Effects of polymorphisms in ovine and caprine prion protein alleles on cell-free conversion
Cell-free conversion of ovine and caprine PrPC variants by scrapie prions. (A) Detection of cell-free converted PrPres. Non-proteinase K (PK) treated wildtype 136A and wildtype IHNRRQ samples are shown in lane 1 and lane 5 respectively. PrPres fragments of 136A (lane 2) and its variants 154H (lane 3) and 171R (lane 4) are depicted. PrPres fragments of caprine IHNRRQ (lane 6) and its variants 142M (lane 7), 143R (lane 8), 146S (lane 9), 146D (lane 10), 151H (lane 11), 211R (lane 12) and 222K (lane 13). The ovine and caprine PrP was detected using monoclonal antibody (mab) P4. Molecular mass marker is indicated on the left. Arrow indicates PrPres fragments. (B) Mean relative conversion efficiencies (± standard error of the mean, SEM) for each set of conversion reaction. Relative conversion rates of ovine PrP variants were calculated in relation to the ovine 136A reference allele and the goat derived variants in relation to the caprine IHNRRQ reference allele. Bars depict the SEM of at least 4 reactions. The differences were analyzed by unpaired student t-test. *: p < 0.05; **: p < 0.01; ***: p < 0.001. ns: not significant.