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Table 2 List of nucleotide sequence differences of CPXV.BR and cloned pBR when compared to the published BR sequence.

From: Recovery of infectious virus from full-length cowpox virus (CPXV) DNA cloned as a bacterial artificial chromosome (BAC)

   A. Changes in coding sequences

Gene (according to NC_003663)

Function

Modification (nt position)

Present in parental virus

Effect

006

ankyrina

C to A (5 038)

no

silent

019

ankyrin

C to T (21 015)

yes

Glu to Lys, not in ankyrin repeats

035

kelch-like

ins. T (36 656)

yes

split protein, resembles situation in other orthopox viruses

079

glutaredoxin (GRX) family

C to T (76 314)

yes

Asp to Asn, polymorphic region of the protein

089

protein G1 (Peptidase)

T to C (87 650)

yes

Asn to Asp, like in 91-3

105

tk

T to C (97 522)

no

silent, PCR error

106

polyA polymerase small SU

(105 299)

no

silent, PCR error

139

poxvirus early transcription factor (VETF)

Insertion : T (142 924)

yes

C-terminal amino acid sequence changed from RAQIN to KSTNKLNN

193

kelch-like

C to T (189 440)

yes

Ser to Phe, polymorphic region of the protein

215

kelch-like

C to T (213 275)

yes

Pro to Ser, polymorphic region of the protein

225

ankyrina

G to T (229 788)

no

silent

   B. Changes in non-coding sequences

Modification (nt position)

Comment

Insertion : TG (8 899-8 900)b

poly-TG repeat, polymorphic in viral DNA

C to T (58 974)

   

Insertion : T (65 922)

poly-T stretch

Insertion : CA (225 926-225 927)b

poly-CA repeat, polymorphic in viral DNA

Insertion : TG (8 899-8 900)b

poly-TG repeat, polymorphic in viral DNA