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Table 2 List of nucleotide sequence differences of CPXV.BR and cloned pBR when compared to the published BR sequence.

From: Recovery of infectious virus from full-length cowpox virus (CPXV) DNA cloned as a bacterial artificial chromosome (BAC)

   A. Changes in coding sequences
Gene (according to NC_003663) Function Modification (nt position) Present in parental virus Effect
006 ankyrina C to A (5 038) no silent
019 ankyrin C to T (21 015) yes Glu to Lys, not in ankyrin repeats
035 kelch-like ins. T (36 656) yes split protein, resembles situation in other orthopox viruses
079 glutaredoxin (GRX) family C to T (76 314) yes Asp to Asn, polymorphic region of the protein
089 protein G1 (Peptidase) T to C (87 650) yes Asn to Asp, like in 91-3
105 tk T to C (97 522) no silent, PCR error
106 polyA polymerase small SU (105 299) no silent, PCR error
139 poxvirus early transcription factor (VETF) Insertion : T (142 924) yes C-terminal amino acid sequence changed from RAQIN to KSTNKLNN
193 kelch-like C to T (189 440) yes Ser to Phe, polymorphic region of the protein
215 kelch-like C to T (213 275) yes Pro to Ser, polymorphic region of the protein
225 ankyrina G to T (229 788) no silent
   B. Changes in non-coding sequences
Modification (nt position) Comment
Insertion : TG (8 899-8 900)b poly-TG repeat, polymorphic in viral DNA
C to T (58 974)    
Insertion : T (65 922) poly-T stretch
Insertion : CA (225 926-225 927)b poly-CA repeat, polymorphic in viral DNA
Insertion : TG (8 899-8 900)b poly-TG repeat, polymorphic in viral DNA