Table 1 Oligonucleotide primers used in this study
Genes | Sequences (5'→3') | Amplicon size (bp) | Annealing temperature (°C) | Accession No. or reference |
|---|---|---|---|---|
IL-1β | Forward: GCAGTGGAGAAGCCGATGAAGA | 199 | 58 | NM_214055 |
Reverse: GGCCAGCCAGCACTAGAGATTTG | ||||
IL-8 | Forward: TTTCTGCAGCTCTCTGTGAGG | 269 | 48 | [30] |
Reverse: CTGCTGTTGTTGTTGCTTCTC | ||||
TNF-α | Forward: CGCATCGCCGTCTCCTACCA | 202 | 58 | NM_214022 |
Reverse: GCCCAGATTCAGCAAAGTCCAGAT | ||||
GAPDH | Forward: GGCTGCCCAGAACATCATCC | 195 | 58 | AF309651 |
Reverse: GACGCCTGCTTCACCACCTTCTTG |