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Figure 5 | Veterinary Research

Figure 5

From: Mechanisms underlying Actinobacillus pleuropneumoniae exotoxin ApxI induced expression of IL-1β, IL-8 and TNF-α in porcine alveolar macrophages

Figure 5

CD18 mediates p38 and JNK activation and proinflammatory cytokine mRNA expression. (A and B) PAMs were pre-incubated with CD18 blocking antibody (Anti-CD18) for 1 h prior to stimulation with 0.5 CU/mL ApxI or control medium (C) for an additional 1 h. Thereafter, cells were lysed and subjected to Western blot analysis with an antibody to phospho-p38 (p-p38) or phospho-JNK (p-JNK). The average intensity of p-p38 or p-JNK isoforms (p46 and p55) was quantified and normalized to the intensity of β-actin (A and B, lower panel). (C) PAMs were pre-incubated with anti-CD18 antibody and stimulated with 0.5 CU/mL ApxI for 2 h, followed by RT-qPCR analysis of cytokine mRNA expression. Data are representative of three independent experiments of at least triplicate determinations.

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