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Figure 2 | Veterinary Research

Figure 2

From: Mechanisms underlying Actinobacillus pleuropneumoniae exotoxin ApxI induced expression of IL-1β, IL-8 and TNF-α in porcine alveolar macrophages

Figure 2

Bioactivity of ApxI is required for induction of proinflammatory cytokine gene expression. PAMs were treated with 0.5 CU/mL of ApxI, heat-inactivated ApxI (Δ), or ApxI pre-incubated with an antiserum raised against recombinant subunit ApxI protein (rA). Medium without ApxI toxin served as a negative control (C). Two hours after stimulation, the total RNA of PAMs was extracted for RT-qPCR analysis. Data are representative of three independent experiments of at least triplicate determinations.

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Veterinary Research

ISSN: 1297-9716