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Figure 1 | Veterinary Research

Figure 1

From: Mechanisms underlying Actinobacillus pleuropneumoniae exotoxin ApxI induced expression of IL-1β, IL-8 and TNF-α in porcine alveolar macrophages

Figure 1

A. pleuropneumoniae exotoxin ApxI induces IL-1β, IL-8 and TNF-α mRNA expression of porcine alveolar macrophages (PAMs) in a concentration- and time-dependent manner. (A) PAMs were stimulated with 0-2 CU/mL ApxI in the presence (w/) or absence (w/o) of polymyxin B (PMB) for 2 h. Total RNA of PAMs was extracted for cDNA synthesis and subjected to RT-qPCR. (B) PAMs were treated with 1 CU/mL ApxI in the presence of PMB for 0-12 h and subjected to RT-qPCR. The expression levels of cytokines were further normalized to corresponding survival rate of PAMs at each time point. Data are representative of three independent experiments of at least triplicate determinations. The error bars are SEM.

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Veterinary Research

ISSN: 1297-9716