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Figure 1 | Veterinary Research

Figure 1

From: Mesenteric lymph node cells from neonates present a prominent IL-12 response to CpG oligodeoxynucleotide via an IL-15 feedback loop of amplification

Figure 1

Comparative IL-12 and IFNγ responses of neonatal and adult cells following TLR agonist stimulation. Neonatal and adult MLN (A, B) and spleen cells (C) were cultured in vitro with 1 μM Ctl-ODN or 1 μM CpG-2006. Supernatants were harvested after 48 h of culture and ELISA was carried out for IL-12 (A, C) and IFNγ (B) secretions. (D) Mouse anti-bovine IL-12 (grey bar) or its isotype control (hatched bar) was added to neonatal MLN cells, at the same time as CpG-2006 or Ctl-ODN. IFNγ secreted into the culture supernatants was determined after 48 h. The mean ± SEM concentration of IFNγ secretion is shown (CpG-2006 - Ctl-ODN). * p ≤ 0.005; ** p ≤ 0.001.

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Veterinary Research

ISSN: 1297-9716