|
4 hours post infection with S. Enteritidis
|
24 hours post infection with S. Enteritidis
|
|---|
| |
wt
|
ΔSPI1
|
n.i.
|
wt
|
ΔSPI1
|
n.i.
|
|
IL-1β
|
74.3 ± 59.7
|
187.5 ± 163.1**
|
0.62 ± 0.28**
|
36.8 ± 13.6
|
238.5 ± 224.5*
|
0.20 ± 0.19*
|
|
IL-8
|
15.7 ± 3.7
|
23.4 ± 9.4
|
0.92 ± 0.57**
|
5.5 ± 4.7
|
17.5 ± 6.4
|
0.19 ± 0.18**
|
|
TNFα
|
57.9 ± 54.9
|
113.8 ± 105.1**
|
1.1 ± 1.0**
|
9.6 ± 7.1
|
15.6 ± 17.0
|
0.20 ± 0.07**
|
|
IL-23α
|
7.6 ± 5.3
|
30.5 ± 22.6**
|
0.05 ± 0.02**
|
4.7 ± 2.6
|
83.1 ± 82.8*
|
0.014 ± 0.007**
|
|
IL-12β
|
0.025 ± 0.026
|
0.043 ± 0.038
|
0.0008 ± 0.0001*
|
0.29 ± 0.31
|
3.01 ± 3.63**
|
0.0009 ± 0.0005**
|
|
TGFβ
|
18.6 ± 6.4
|
18.7 ± 6.8
|
11.2 ± 2.1
|
15.2 ± 7.2
|
25.4 ± 18.3
|
12.3 ± 5.6
|
|
IL-18
|
0.56 ± 0.34
|
0.72 ± 0.39*
|
0.16 ± 0.08
|
0.66 ± 0.38
|
1.13 ± 0.49
|
0.60 ± 0.58
|
|
GM-CSF
|
0.19 ± 0.15
|
0.32 ± 0.19*
|
0.02 ± 0.03**
|
0.37 ± 0.27
|
1.92 ± 1.64**
|
0.015 ± 0.020**
|
|
IL-6
|
0.27 ± 0.37
|
0.92 ± 1.35
|
0.0012 ± 0.0011
|
0.23 ± 0.34
|
1.79 ± 2.88*
|
n.d.
|
|
NRAMP1
|
36.8 ± 20.0
|
32.9 ± 21.1
|
25.8 ± 12.9*
|
29.6 ± 17.1
|
67.9 ± 48.5*
|
18.0 ± 7.7
|
|
SOD1
|
5.9 ± 2.5
|
6.3 ± 3.0
|
4.9 ± 2.3
|
3.7 ± 1.6
|
7.5 ± 5.5
|
3.4 ± 1.4
|
|
phox47
|
4.7 ± 1.4
|
6.5 ± 1.7*
|
5.5 ± 1.7
|
2.9 ± 0.5
|
4.2 ± 1.1*
|
4.2 ± 2.0
|
|
iNOS
|
0.012 ± 0.005
|
0.064 ± 0.035**
|
0.0015 ± 0.0004**
|
0.0055 ± 0.0061
|
0.026 ± 0.039
|
0.0025 ± 0.0006
|
- n.d. - not detected, expression too low to be detected by RT PCR. * p < 0.05, ** p < 0.01 when compared to the PAMs infected with the wild-type strain
