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Table 3 Expression of cytokine or immune relevant genes in non-infected PAMs (n.i.) and PAMs 4 and 24 h post infection with the wild-type S. Enteritidis and ΔSPI1 mutant.

From: SPI-1-encoded type III secretion system of Salmonella enterica is required for the suppression of porcine alveolar macrophage cytokine expression

4 hours post infection with S. Enteritidis 24 hours post infection with S. Enteritidis
  wt ΔSPI1 n.i. wt ΔSPI1 n.i.
IL-1β 74.3 ± 59.7 187.5 ± 163.1** 0.62 ± 0.28** 36.8 ± 13.6 238.5 ± 224.5* 0.20 ± 0.19*
IL-8 15.7 ± 3.7 23.4 ± 9.4 0.92 ± 0.57** 5.5 ± 4.7 17.5 ± 6.4 0.19 ± 0.18**
TNFα 57.9 ± 54.9 113.8 ± 105.1** 1.1 ± 1.0** 9.6 ± 7.1 15.6 ± 17.0 0.20 ± 0.07**
IL-23α 7.6 ± 5.3 30.5 ± 22.6** 0.05 ± 0.02** 4.7 ± 2.6 83.1 ± 82.8* 0.014 ± 0.007**
IL-12β 0.025 ± 0.026 0.043 ± 0.038 0.0008 ± 0.0001* 0.29 ± 0.31 3.01 ± 3.63** 0.0009 ± 0.0005**
TGFβ 18.6 ± 6.4 18.7 ± 6.8 11.2 ± 2.1 15.2 ± 7.2 25.4 ± 18.3 12.3 ± 5.6
IL-18 0.56 ± 0.34 0.72 ± 0.39* 0.16 ± 0.08 0.66 ± 0.38 1.13 ± 0.49 0.60 ± 0.58
GM-CSF 0.19 ± 0.15 0.32 ± 0.19* 0.02 ± 0.03** 0.37 ± 0.27 1.92 ± 1.64** 0.015 ± 0.020**
IL-6 0.27 ± 0.37 0.92 ± 1.35 0.0012 ± 0.0011 0.23 ± 0.34 1.79 ± 2.88* n.d.
NRAMP1 36.8 ± 20.0 32.9 ± 21.1 25.8 ± 12.9* 29.6 ± 17.1 67.9 ± 48.5* 18.0 ± 7.7
SOD1 5.9 ± 2.5 6.3 ± 3.0 4.9 ± 2.3 3.7 ± 1.6 7.5 ± 5.5 3.4 ± 1.4
phox47 4.7 ± 1.4 6.5 ± 1.7* 5.5 ± 1.7 2.9 ± 0.5 4.2 ± 1.1* 4.2 ± 2.0
iNOS 0.012 ± 0.005 0.064 ± 0.035** 0.0015 ± 0.0004** 0.0055 ± 0.0061 0.026 ± 0.039 0.0025 ± 0.0006
  1. n.d. - not detected, expression too low to be detected by RT PCR. * p < 0.05, ** p < 0.01 when compared to the PAMs infected with the wild-type strain