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Table 3 Expression of cytokine or immune relevant genes in non-infected PAMs (n.i.) and PAMs 4 and 24 h post infection with the wild-type S. Enteritidis and ΔSPI1 mutant.

From: SPI-1-encoded type III secretion system of Salmonella enterica is required for the suppression of porcine alveolar macrophage cytokine expression

4 hours post infection with S. Enteritidis

24 hours post infection with S. Enteritidis

 

wt

ΔSPI1

n.i.

wt

ΔSPI1

n.i.

IL-1β

74.3 ± 59.7

187.5 ± 163.1**

0.62 ± 0.28**

36.8 ± 13.6

238.5 ± 224.5*

0.20 ± 0.19*

IL-8

15.7 ± 3.7

23.4 ± 9.4

0.92 ± 0.57**

5.5 ± 4.7

17.5 ± 6.4

0.19 ± 0.18**

TNFα

57.9 ± 54.9

113.8 ± 105.1**

1.1 ± 1.0**

9.6 ± 7.1

15.6 ± 17.0

0.20 ± 0.07**

IL-23α

7.6 ± 5.3

30.5 ± 22.6**

0.05 ± 0.02**

4.7 ± 2.6

83.1 ± 82.8*

0.014 ± 0.007**

IL-12β

0.025 ± 0.026

0.043 ± 0.038

0.0008 ± 0.0001*

0.29 ± 0.31

3.01 ± 3.63**

0.0009 ± 0.0005**

TGFβ

18.6 ± 6.4

18.7 ± 6.8

11.2 ± 2.1

15.2 ± 7.2

25.4 ± 18.3

12.3 ± 5.6

IL-18

0.56 ± 0.34

0.72 ± 0.39*

0.16 ± 0.08

0.66 ± 0.38

1.13 ± 0.49

0.60 ± 0.58

GM-CSF

0.19 ± 0.15

0.32 ± 0.19*

0.02 ± 0.03**

0.37 ± 0.27

1.92 ± 1.64**

0.015 ± 0.020**

IL-6

0.27 ± 0.37

0.92 ± 1.35

0.0012 ± 0.0011

0.23 ± 0.34

1.79 ± 2.88*

n.d.

NRAMP1

36.8 ± 20.0

32.9 ± 21.1

25.8 ± 12.9*

29.6 ± 17.1

67.9 ± 48.5*

18.0 ± 7.7

SOD1

5.9 ± 2.5

6.3 ± 3.0

4.9 ± 2.3

3.7 ± 1.6

7.5 ± 5.5

3.4 ± 1.4

phox47

4.7 ± 1.4

6.5 ± 1.7*

5.5 ± 1.7

2.9 ± 0.5

4.2 ± 1.1*

4.2 ± 2.0

iNOS

0.012 ± 0.005

0.064 ± 0.035**

0.0015 ± 0.0004**

0.0055 ± 0.0061

0.026 ± 0.039

0.0025 ± 0.0006

  1. n.d. - not detected, expression too low to be detected by RT PCR. * p < 0.05, ** p < 0.01 when compared to the PAMs infected with the wild-type strain