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Table 2 Expression of cytokine or immune relevant genes in non-infected PAMs, and in PAMs after 4 and 24 h exposure to LPS (1 μg/mL), wild-type S. Typhimurium and ΔSPI1 mutant.

From: SPI-1-encoded type III secretion system of Salmonella enterica is required for the suppression of porcine alveolar macrophage cytokine expression

  4 hours 24 hours
  wt ΔSPI1 LPS non-infected wt ΔSPI1 LPS non-infected
IL-1β 153 ± 99.8 529 ± 319** 276 ± 104 2.05 ± 1.75** 19.5 ± 12.1 114 ± 83.8** 34.5 ± 25.7 0.16 ± 0.081**
IL-8 91.4 ± 76.2 232 ± 198** 179 ± 57.9 4.79 ± 1.68** 34.2 ± 27.9 140 ± 109** 62.4 ± 24.3 1.32 ± 1.17**
TNFα 79.8 ± 31.4 310 ± 116** 40.5 ± 9.25 2.19 ± 1.20** 3.33 ± 1.27 5.21 ± 2.14* 1.10 ± 0.24 0.33 ± 0.18**
IL-23α 22.0 ± 16.5 123 ± 87.4** 32.7 ± 15.5 0.24 ± 0.21** 9.26 ± 2.89 61.0 ± 22.9** 11.6 ± 6.24 0.066 ± 0.029**
GM-CSF 0.62 ± 0.56 2.63 ± 2.72** 0.97 ± 0.49 0.045 ± 0.018* 1.50 ± 0.53 5.14 ± 5.40** 1.61 ± 1.19 0.013 ± 0.0039*
IL-12β 0.15 ± 0.17 0.19 ± 0.20 0.39 ± 0.29 0.052 ± 0.048 0.32 ± 0.22 0.75 ± 0.46* 0.060 ± 0.046 0.0031 ± 0.0019**
IL-18 0.40 ± 0.25 0.51 ± 0.31* 0.54 ± 0.13 0.16 ± 0.041* 0.47 ± 0.15 0.68 ± 0.35 0.95 ± 0.47 0.16 ± 0.041**
IL-6 0.11 ± 0.13 0.35 ± 0.42** 0.21 ± 0.21 0.0027 ± 0.0028** 0.11 ± 0.16 0.11 ± 0.17 0.094 ± 0.092 0.0034 ± 0.0041**
TGFβ 32.0 ± 17.4 34.2 ± 18.6 37.1 ± 9.53 26.7 ± 8.57 42.1 ± 8.29 34.8 ± 7.04 25.5 ± 4.86 20.6 ± 10.1**
NRAMP1 60.7 ± 17.0 61.7 ± 13.8 47.9 ± 8.72 64.3 ± 14.9 85.7 ± 43.4 92.2 ± 37.1 46.9 ± 1.91 43.6 ± 9.13*
SOD1 14.8 ± 4.79 15.7 ± 2.29 18.4 ± 6.38 15.8 ± 2.70 14.7 ± 6.30 11.5 ± 2.21 11.5 ± 1.48 9.63 ± 1.89
phox47 5.08 ± 3.25 7.05 ± 3.98 3.44 ± 1.87 7.98 ± 3.70 3.52 ± 2.57 4.22 ± 3.30 2.44 ± 1.07 4.40 ± 1.98
iNOS 0.019 ± 0.015 0.051 ± 0.037* 0.040 ± 0.014 0.0035 ± 0.0029 0.0030 ± 0.0018 0.0026 ± 0.0016 0.0027 ± 0.0018 0.0031 ± 0.0025
  1. * p < 0.05, ** p < 0.01 when compared to the PAMs infected with the wild-type strain