4 hours | 24 hours | |||||||
---|---|---|---|---|---|---|---|---|
wt | ΔSPI1 | LPS | non-infected | wt | ΔSPI1 | LPS | non-infected | |
IL-1β | 153 ± 99.8 | 529 ± 319** | 276 ± 104 | 2.05 ± 1.75** | 19.5 ± 12.1 | 114 ± 83.8** | 34.5 ± 25.7 | 0.16 ± 0.081** |
IL-8 | 91.4 ± 76.2 | 232 ± 198** | 179 ± 57.9 | 4.79 ± 1.68** | 34.2 ± 27.9 | 140 ± 109** | 62.4 ± 24.3 | 1.32 ± 1.17** |
TNFα | 79.8 ± 31.4 | 310 ± 116** | 40.5 ± 9.25 | 2.19 ± 1.20** | 3.33 ± 1.27 | 5.21 ± 2.14* | 1.10 ± 0.24 | 0.33 ± 0.18** |
IL-23α | 22.0 ± 16.5 | 123 ± 87.4** | 32.7 ± 15.5 | 0.24 ± 0.21** | 9.26 ± 2.89 | 61.0 ± 22.9** | 11.6 ± 6.24 | 0.066 ± 0.029** |
GM-CSF | 0.62 ± 0.56 | 2.63 ± 2.72** | 0.97 ± 0.49 | 0.045 ± 0.018* | 1.50 ± 0.53 | 5.14 ± 5.40** | 1.61 ± 1.19 | 0.013 ± 0.0039* |
IL-12β | 0.15 ± 0.17 | 0.19 ± 0.20 | 0.39 ± 0.29 | 0.052 ± 0.048 | 0.32 ± 0.22 | 0.75 ± 0.46* | 0.060 ± 0.046 | 0.0031 ± 0.0019** |
IL-18 | 0.40 ± 0.25 | 0.51 ± 0.31* | 0.54 ± 0.13 | 0.16 ± 0.041* | 0.47 ± 0.15 | 0.68 ± 0.35 | 0.95 ± 0.47 | 0.16 ± 0.041** |
IL-6 | 0.11 ± 0.13 | 0.35 ± 0.42** | 0.21 ± 0.21 | 0.0027 ± 0.0028** | 0.11 ± 0.16 | 0.11 ± 0.17 | 0.094 ± 0.092 | 0.0034 ± 0.0041** |
TGFβ | 32.0 ± 17.4 | 34.2 ± 18.6 | 37.1 ± 9.53 | 26.7 ± 8.57 | 42.1 ± 8.29 | 34.8 ± 7.04 | 25.5 ± 4.86 | 20.6 ± 10.1** |
NRAMP1 | 60.7 ± 17.0 | 61.7 ± 13.8 | 47.9 ± 8.72 | 64.3 ± 14.9 | 85.7 ± 43.4 | 92.2 ± 37.1 | 46.9 ± 1.91 | 43.6 ± 9.13* |
SOD1 | 14.8 ± 4.79 | 15.7 ± 2.29 | 18.4 ± 6.38 | 15.8 ± 2.70 | 14.7 ± 6.30 | 11.5 ± 2.21 | 11.5 ± 1.48 | 9.63 ± 1.89 |
phox47 | 5.08 ± 3.25 | 7.05 ± 3.98 | 3.44 ± 1.87 | 7.98 ± 3.70 | 3.52 ± 2.57 | 4.22 ± 3.30 | 2.44 ± 1.07 | 4.40 ± 1.98 |
iNOS | 0.019 ± 0.015 | 0.051 ± 0.037* | 0.040 ± 0.014 | 0.0035 ± 0.0029 | 0.0030 ± 0.0018 | 0.0026 ± 0.0016 | 0.0027 ± 0.0018 | 0.0031 ± 0.0025 |