| |
4 hours
|
24 hours
|
|---|
| |
wt
|
ΔSPI1
|
LPS
|
non-infected
|
wt
|
ΔSPI1
|
LPS
|
non-infected
|
|
IL-1β
|
153 ± 99.8
|
529 ± 319**
|
276 ± 104
|
2.05 ± 1.75**
|
19.5 ± 12.1
|
114 ± 83.8**
|
34.5 ± 25.7
|
0.16 ± 0.081**
|
|
IL-8
|
91.4 ± 76.2
|
232 ± 198**
|
179 ± 57.9
|
4.79 ± 1.68**
|
34.2 ± 27.9
|
140 ± 109**
|
62.4 ± 24.3
|
1.32 ± 1.17**
|
|
TNFα
|
79.8 ± 31.4
|
310 ± 116**
|
40.5 ± 9.25
|
2.19 ± 1.20**
|
3.33 ± 1.27
|
5.21 ± 2.14*
|
1.10 ± 0.24
|
0.33 ± 0.18**
|
|
IL-23α
|
22.0 ± 16.5
|
123 ± 87.4**
|
32.7 ± 15.5
|
0.24 ± 0.21**
|
9.26 ± 2.89
|
61.0 ± 22.9**
|
11.6 ± 6.24
|
0.066 ± 0.029**
|
|
GM-CSF
|
0.62 ± 0.56
|
2.63 ± 2.72**
|
0.97 ± 0.49
|
0.045 ± 0.018*
|
1.50 ± 0.53
|
5.14 ± 5.40**
|
1.61 ± 1.19
|
0.013 ± 0.0039*
|
|
IL-12β
|
0.15 ± 0.17
|
0.19 ± 0.20
|
0.39 ± 0.29
|
0.052 ± 0.048
|
0.32 ± 0.22
|
0.75 ± 0.46*
|
0.060 ± 0.046
|
0.0031 ± 0.0019**
|
|
IL-18
|
0.40 ± 0.25
|
0.51 ± 0.31*
|
0.54 ± 0.13
|
0.16 ± 0.041*
|
0.47 ± 0.15
|
0.68 ± 0.35
|
0.95 ± 0.47
|
0.16 ± 0.041**
|
|
IL-6
|
0.11 ± 0.13
|
0.35 ± 0.42**
|
0.21 ± 0.21
|
0.0027 ± 0.0028**
|
0.11 ± 0.16
|
0.11 ± 0.17
|
0.094 ± 0.092
|
0.0034 ± 0.0041**
|
|
TGFβ
|
32.0 ± 17.4
|
34.2 ± 18.6
|
37.1 ± 9.53
|
26.7 ± 8.57
|
42.1 ± 8.29
|
34.8 ± 7.04
|
25.5 ± 4.86
|
20.6 ± 10.1**
|
|
NRAMP1
|
60.7 ± 17.0
|
61.7 ± 13.8
|
47.9 ± 8.72
|
64.3 ± 14.9
|
85.7 ± 43.4
|
92.2 ± 37.1
|
46.9 ± 1.91
|
43.6 ± 9.13*
|
|
SOD1
|
14.8 ± 4.79
|
15.7 ± 2.29
|
18.4 ± 6.38
|
15.8 ± 2.70
|
14.7 ± 6.30
|
11.5 ± 2.21
|
11.5 ± 1.48
|
9.63 ± 1.89
|
|
phox47
|
5.08 ± 3.25
|
7.05 ± 3.98
|
3.44 ± 1.87
|
7.98 ± 3.70
|
3.52 ± 2.57
|
4.22 ± 3.30
|
2.44 ± 1.07
|
4.40 ± 1.98
|
|
iNOS
|
0.019 ± 0.015
|
0.051 ± 0.037*
|
0.040 ± 0.014
|
0.0035 ± 0.0029
|
0.0030 ± 0.0018
|
0.0026 ± 0.0016
|
0.0027 ± 0.0018
|
0.0031 ± 0.0025
|
- * p < 0.05, ** p < 0.01 when compared to the PAMs infected with the wild-type strain
