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Table 2 Expression of cytokine or immune relevant genes in non-infected PAMs, and in PAMs after 4 and 24 h exposure to LPS (1 μg/mL), wild-type S. Typhimurium and ΔSPI1 mutant.

From: SPI-1-encoded type III secretion system of Salmonella enterica is required for the suppression of porcine alveolar macrophage cytokine expression

 

4 hours

24 hours

 

wt

ΔSPI1

LPS

non-infected

wt

ΔSPI1

LPS

non-infected

IL-1β

153 ± 99.8

529 ± 319**

276 ± 104

2.05 ± 1.75**

19.5 ± 12.1

114 ± 83.8**

34.5 ± 25.7

0.16 ± 0.081**

IL-8

91.4 ± 76.2

232 ± 198**

179 ± 57.9

4.79 ± 1.68**

34.2 ± 27.9

140 ± 109**

62.4 ± 24.3

1.32 ± 1.17**

TNFα

79.8 ± 31.4

310 ± 116**

40.5 ± 9.25

2.19 ± 1.20**

3.33 ± 1.27

5.21 ± 2.14*

1.10 ± 0.24

0.33 ± 0.18**

IL-23α

22.0 ± 16.5

123 ± 87.4**

32.7 ± 15.5

0.24 ± 0.21**

9.26 ± 2.89

61.0 ± 22.9**

11.6 ± 6.24

0.066 ± 0.029**

GM-CSF

0.62 ± 0.56

2.63 ± 2.72**

0.97 ± 0.49

0.045 ± 0.018*

1.50 ± 0.53

5.14 ± 5.40**

1.61 ± 1.19

0.013 ± 0.0039*

IL-12β

0.15 ± 0.17

0.19 ± 0.20

0.39 ± 0.29

0.052 ± 0.048

0.32 ± 0.22

0.75 ± 0.46*

0.060 ± 0.046

0.0031 ± 0.0019**

IL-18

0.40 ± 0.25

0.51 ± 0.31*

0.54 ± 0.13

0.16 ± 0.041*

0.47 ± 0.15

0.68 ± 0.35

0.95 ± 0.47

0.16 ± 0.041**

IL-6

0.11 ± 0.13

0.35 ± 0.42**

0.21 ± 0.21

0.0027 ± 0.0028**

0.11 ± 0.16

0.11 ± 0.17

0.094 ± 0.092

0.0034 ± 0.0041**

TGFβ

32.0 ± 17.4

34.2 ± 18.6

37.1 ± 9.53

26.7 ± 8.57

42.1 ± 8.29

34.8 ± 7.04

25.5 ± 4.86

20.6 ± 10.1**

NRAMP1

60.7 ± 17.0

61.7 ± 13.8

47.9 ± 8.72

64.3 ± 14.9

85.7 ± 43.4

92.2 ± 37.1

46.9 ± 1.91

43.6 ± 9.13*

SOD1

14.8 ± 4.79

15.7 ± 2.29

18.4 ± 6.38

15.8 ± 2.70

14.7 ± 6.30

11.5 ± 2.21

11.5 ± 1.48

9.63 ± 1.89

phox47

5.08 ± 3.25

7.05 ± 3.98

3.44 ± 1.87

7.98 ± 3.70

3.52 ± 2.57

4.22 ± 3.30

2.44 ± 1.07

4.40 ± 1.98

iNOS

0.019 ± 0.015

0.051 ± 0.037*

0.040 ± 0.014

0.0035 ± 0.0029

0.0030 ± 0.0018

0.0026 ± 0.0016

0.0027 ± 0.0018

0.0031 ± 0.0025

  1. * p < 0.05, ** p < 0.01 when compared to the PAMs infected with the wild-type strain