Rectal temperature (A) and rumen motility rate (B) of 9 primiparous cows challenged intramammarily in 18 quarters (left quarters) with 1 × 104 cfu E. coli strain P4, serotype O32:H37 at post infusion hour (PIH) 0 during early lactation. Means (± SEM) are shown. The clinical symptoms were scored according to Vangroenweghe et al. .* P < 0.05, ** P < 0.01, ***P < 0.001: significance of difference from the control value.