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Figure 2 | Veterinary Research

Figure 2

From: Susceptibility of in vitro produced hatched bovine blastocysts to infection with bluetongue virus serotype 8

Figure 2

Blastocysts examined for apoptosis at different time points post-exposure to BTV-8. Column 1: Hoechst staining of nuclei; Column 2: terminal deoxynucleotidyl transferase (TdT) fluorescein-dUTP Nick End Labelling (TUNEL) for apoptosis detection and Column 3: indirect immunofluorescent staining of viral BTV-8 antigen with a Texas-Red labelled secondary antibody. Column 4: overlays of the three staining procedures. Row A: BTV-8 exposed blastocyst of excellent morphological quality at 48 hpe showing BTV-8 viral antigen in all its cells. Row B: BTV-8 positive blastocyst at 48 hpe showing degeneration and a high proportion of apoptotic cells. Row C: BTV-8 positive blastocyst at 72 hpe with excellent morphological quality. Row D: BTV-8 positive blastocysts at 72 hpe which shows no colocalisation of apoptosis and BTV-8 infection, and which has an excellent morphology.

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Veterinary Research

ISSN: 1297-9716