Figure 1From: Susceptibility of in vitro produced hatched bovine blastocysts to infection with bluetongue virus serotype 8Blastocysts examined by immunofluorescence microscopy at different time points post exposure to BTV-8. Column 1: propidium iodide (PI) staining of nuclei; Column 2: indirect fluorescein (FITC) labelled detection of BTV-8 antigen and Column 3: overlay of PI and FITC staining. Row A: blastocyst negative for antigen at 48 h post exposure (hpe). Row B: blastocyst negative for antigen at 60 hpe. Row C: blastocyst positive for antigen at 48 hpe. Row D: blastocyst completely positive for antigen at 72 hpe. Row E: blastocyst positive for antigen at 72 hpe. Row F: blastocyst positive for antigen at 96 hpe.Back to article page