Changes in HSP gene and protein expression in natural scrapie with brain damage

Table 1 Genes analysed and real time RT-PCR conditions

Genes	GenBank¹	Species²	Primers (5'→ 3')³	bp⁴	PCR conditions⁵
					Ta	[nM]	r ²	Slope
HSP27	BT021550	Bovine	F: TGGCGCGTGTCCCTGGA	80	60	300	0.990	-3.33
			R: GTGATCTCCACCACGCC			300
HSP72	AY662497	Bovine	F: ACCCGCAGAACACGGTGTT	119	60	900	0.995	-3.33
			R: AGGCTTGTCTCCGTCGTTGA			900
HSP73	NM_174345	Bovine	F: CAACCTGCTTGGCAAGTTTGA	108	60	900	0.990	-3.20
			R: GAAACATTGAGGATGCCATTGG			900
HSP90	[30]	Ovine	F: AGTCTGGAGGATCCCCAGACA	78	60	300	0.994	-3.32
			R: GGGTCATCCTCGTCAATACCA			300

¹ GenBank accession numbers of the sequences used for primer design.
² Species of origin of the sequences.
³ Primers (F: Forward and R: Reverse) used for the gene amplification.
⁴ Length of the amplicon in base pairs (bp).
⁵ Real-time RT-PCR conditions for gene expression analyses: annealing temperature (Ta), primer concentration ([nM]), correlation coefficient (r²) and slope of the standard curve.

ISSN: 1297-9716