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Figure 5 | Veterinary Research

Figure 5

From: Theileria annulata-transformed cell lines are efficient antigen-presenting cells for in vitro analysis of CD8 T cell responses to bovine herpesvirus-1

Figure 5

Proliferative responses of CD8 T cells from BHV-1 immune animals to autologous T. annulata -transformed cells infected with BHV-1. PBMC from two immune animals (03430 and 03158) were stimulated with autologous T. annulata transformed cells (Ta) or the same cells infected with BHV-1 (Ta BHV). Proliferation of CD8 T cells was examined by pre-incubation of the PBMC with CFSE and analysis of the cells after 7 days by two-colour immunofluorescence to detect CSFE and CD8. The percentages of CD8+ cells in the cultures showing loss of CSFE staining (indicating cell division) and those retaining CFSE staining are shown in the lower right and upper right quadrants respectively.

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Veterinary Research

ISSN: 1297-9716