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Table 1 Overview of samples collected during the trial.

From: Intestinal infection following aerosol challenge of calves with Mycobacterium avium subspecies paratuberculosis

Type of sample Total Frequency Pool Analysis Reason
   F0;
before inoculation
3 calves per pool:
1: oral
2: no challenge
3a&b: T-tracheal
4a&b: Nasal
  Faecal MAP shedding before infection
Faeces 13 per calf Week 1 F1-3 pi
Week 2 F4-6 pi
Week 3 F7-9 pi;
Each of 3 days following weekly inoculation.
3 samples of 2 calves per pool:
1a&b: oral
2a&b: no challenge
3a, b&c: T-tracheal
4a, b&c: Nasal
Liquid MAP culture with confirmation IS900 PCR Passive faecal MAP shedding after inoculation
   Week 6 F10 pi 3 calves per pool:
1: oral
2: no challenge
3a&b: T-tracheal
4a&b: Nasal
  Faecal MAP Shedding
   Week 9 F11 pi Individually   
   Week 12 F12 pi Individually   Faecal MAP Shedding
Dust 18; at each stall Week 11 pi Individually Liquid MAP culture with confirmation IS900 PCR Environmental contamination with MAP
Serum 2 per calf Week 0 pi
Week 12 pi
Individually Commercial JD-ELISA B-cell activation& seroconversion
Whole blood 1 per calf Week 12 pi Individually Bovigam® interferon gamma essay T-cell sensitisation
Tissue 18 (19*) per calf W 12 pi Individually Liquid MAP culture with confirmation IS900 PCR Tissue colonisation with MAP
  1. Overview of the samples collected. Frequencies and time points of collection and tests used for analysis during the 12 week trial are described. Additionally, the reason for sampling is indicated. F = faecal sample, pi = post inoculation, T-tracheal = transtracheal. *an additional piece of lung was sampled from one calf.