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Figure 6 | Veterinary Research

Figure 6

From: Immunobiotic lactic acid bacteria beneficially regulate immune response triggered by poly(I:C) in porcine intestinal epithelial cells

Figure 6

Effect of lactobacilli in porcine intestinal epithelial (PIE) and immune cell co-cultures. PIE cells were co-cultured with ileal PP immunocompetent cells, pre-treated with L. casei MEP221106 or L. casei MEP221103 for 48 h and then stimulated with poly(I:C). Cytokines were studied at the times indicated post-stimulation. (A) Expression of IFN-α, IFN-β, IL-6, MCP-1 and TNF-α in PIE cells. (B) Expression of IFN-α, IFN-β, IFN-γ, IL-12p40, TNF-α, IL-1β, IL-2, IL-6, IL-10 and TGF-β in immune cells. Cytokine mRNA levels were calibrated by the swine β-actin level and normalized by common logarithmic transformation. Values represent means and error bars indicate the standard deviations. The results represent three independent experiments. Values with different superscript letters in the same time post-poly(I:C) stimulation are significantly different at the 5% level.

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